engleski

Evaluation of antioxidative potential of broccoli, cauliflower and kale seedlings

Cruciferous plants (family Brassicaceae) are known as a great source of healthy phytochemicals. In recent years, focus shifted from mature plants to seedlings of cruciferous plants. They emerged as a rich source of phenolic compounds and glucosinolates. However, detailed analysis of their bioactivity is still lacking. Considering a rise in prevalence of chronic illnesses due to modern lifestyle, plant foods are considered as food supplements because of the richness in healthy compounds. Of particular interest are phytochemicals with high antioxidative capacity. In this study, antioxidative potential of broccoli (Brassica oleracea var. italica), cauliflower (B. oleracea var. botrytis) and kale (B. oleracea var. acephala) seedlings extracts was assessed by ABTS, DPPH and FRAP assays. Results of the FRAP assay revealed that kale seedlings extract had the highest antioxidative capacity of all three extracts, with no difference between broccoli and cauliflower extracts. ABTS assay revealed that kale and cauliflower seedlings extract had higher antioxidative capacity than broccoli seedlings extract. DPPH assay did not show statistically significant difference in antioxidative capacity of three assayed extracts. Considering that two assays revealed that kale extract had the highest antioxidative capacity, this extract should be further evaluated for its bioactivity. Nevertheless, all three Brassica seedlings extracts showed high antioxidative capacity in vitro. Therefore, their intake as food supplements with high antioxidative potential is highly recommended. Further studies should aim to identify compounds from seedlings extract that contribute to antioxidative capacity of extracts the most, and evaluate their antioxidative properties in vivo.

ABTS, Brassica, DPPH, FRAP, microgreens, phenolics

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