engleski

Different conjugation strategies of gold nanoparticles with anti-SARS-CoV-2 antibodies for ultrasensitive detection of coronavirus

In recent years, gold nanoparticles (AuNPs) have attracted vast interest in biosensing applications. Their unique functional and surface plasmon resonance properties, coupled with possibility to bound various biomolecules (e.g., antibodies, DNA, and RNA aptamers) may significantly enhance diagnostic features. Therefore, functionalizing AuNPs with antibodies could be a new approach for detecting COVID-19 caused by SARS-CoV-2 virus (1, 2). Here we present three conjugation strategies in order to assure efficient binding of monoclonal anti-SARS-CoV-2 antibodies to AuNPs in the oriented way: a) direct conjugation of antibodies by electrostatic interactions or physical adsorption on AuNPs, b) conjugation mediated by glycopeptide-functionalized AuNPs using peptidoglycan monomer, GlcNAc- MurNAc-L-Ala-D-isoGln- mesoDAP(NH2)-D-Ala-D-Ala (PGM)) and c) covalent conjugation using EDC/NHS method. The AuNPsantibodies conjugates were characterized by various spectroscopic and electrophoretic techniques. Additionally, gold nanoparticles labelled with secondary antibodies (Immunogold) were used to determine the amount of available epitopes of the primary monoclonal antibodies on the metal surface. Preliminary results showed that binding efficiency of antibody-AuNPs conjugate can be improved using the covalent binding strategy with their high potential for use in SARS-CoV-2 virus detection.

Gold nanoparticles, SARS-CoV-2 virus

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