engleski

Biocompatibility assessment of selenium nanoparticles as potential drug delivery system

Parkinson’s disease (PD) is neurological disorder characterized by the loss of dopaminergic neurons. Because of that, dopamine levels are reduced and main consequences are bradykinesia, rigidity, tremor and depression (1). Levodopa is the most common therapy for PD but long-term treatment can have a lot of side effects. In order to that, drug delivery system needs to be designed for improving targeting ability. Selenium nanoparticles (SeNPs) constitute an attractive carrier platform because of their antioxidant properties and neuroprotective effects (2). This study aimed to develop drug delivery system consisted of selenium nanoparticles coated with polyvinylpyrrolidone (PVP) and polysorbate 20 (Tween 20). Differently coated SeNPs were prepared via reduction of sodium selenite by L-ascorbic acid. Synthesized nanoparticles were characterized with dynamic light scattering (DLS) and transmission electron microscopy (TEM) for determining their shape and size. In vitro model of blood-brain barrier (BBB), hBEC-5i cell line, was used in all experiments. Cell viability testing, oxidative stress response and permeability evaluation of BBB for SeNPs were used in biocompatibility assessment. For oxidative stress response experiments 3 different assays were used: dichlorodihydrofluorescein diacetate (DCFH- DA), dihydroethidium (DHE) and rhodamine 123 (Rh123). In transwell experiments cells were treated with SeNPs alone and in combination with L-dopa. Concentration of Se in all compartments (donor well, acceptor well, cells, gelatin) was analyzed by Graphite Furnace Atomic Absorption Spectroscopy (GFAAS) while L-dopa concentration was determined by commercial ELISA kit. Results showed that SeNPs in concentration of 1 mg Se/L did not reduce the cell viability significantly and that SeTween NPs have lower level of ROS production than SePVP. Transwell experiments resulted in low permeability for both SeNPs type but in vitro assays that were used in this study were not suitable for evaluating the BBB permeability of SeNPs because of their sticky properties.

Parkinson's disease, selenium nanoparticles, permeability

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