engleski

Preferences of different polyphenol groups of P. spinosa L. flower extract toward organ bioaccumulation in in vivo model of absorption in C57BL/6 mice

Polyphenol (PPH) organ distribution may explicite their physiological efficacy. Water extract of blackthorn (Prunus spinosa L.) flower (PSE) was used to model the tissue distribution of PPH in C57BL/6 mice following oral acute and repeated (subchronic 28 days) administration of 25 mg/kg bw of total PPH. 32 individual PPH's were measured by UPLC-MS (15 min, 30 min, 1h, 3h, 6h, 24h in acute and 1st, 7th, 14th, 21th, 28th day in subchronic treatment).Highest present PPH in the PSE were not necessarily the highest bioavialable in the tissues. Repeated treatment increased the number of significantly higher PPHv Cmax/AUCalast, innn PSE treated animals vs. control, by +6% in the intestine, +45.5% in the liver. +16.0% in kidney and +32% in the brain. Intestine had preference for bioaccumulation of ferulloylquinic acid and kaempherol-pentoside, liver for kaempherol- pentosid and quercetin-3-O-rutinosid, kidney for ferullic acid and quercetin-rutinoside and brain bioacumullated quercetin-rhamnoside and quercetin- rutinoside.

bioavailability ; Prunus spinosa L. ; polyphenol compounds

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