engleski

Micro-extraction by packed sorbent (MEPS) of andrographolide's derivates in human plasma

Andrographolides are diterpenoids found in the plant of Andrographis paniculata (Burm.f.) Nees (Acanthaceae) and widely used in traditional medicine, exhibiting anti-inflammatory property. In this research, a novel micro-extraction by packed sorbent (MEPS) procedure for sample preparation was examined with the aim of extraction of pharmacological active andrographolide, neoandrographolide and 14- deoxyandrographolide from plasma samples. The analysis of samples was performed using developed and validated high-performance liquid chromatography with diode array detection (HPLC/DAD) method. The validation results showed acceptable linearity of the method (R2>0.99) for all analytes within the calibration range. Acceptable accuracy and precision of the method were indicated by recovery of 91–100% and Relative Standard Deviation (RSD) values below 7.6%.The extraction recovery was found to be between 91.04 and 100.34% with RSD values not higher than 11.5%. The limit of quantification was found to be between 62 and 322 ng/mL while the limit of detection was found between 18 and 97 ng/mL. Short-term (1 - 3 days at room temperature) and long-term stability (3 days at -80 °C) were also tested and showed no significant difference (RSDs were lower than 14.8%). The development of this fast and easy MEPS pretreatment procedure granted agreeable validation results and it represents a bioanalytical improvement (cheaper, greener and less time-consuming) over classical solid-phase extraction methods.

MEPS, human plasma, HPLC

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