engleski

Effect of moderate white wine consumption on the expression of HSP70, GPx, CAT and NQO1 in rat cardiomyocytes

Introduction: There is strong epidemiological evidence that moderate consumption of wine has beneficial effects on human health, particularly on cardiovascular system. Increase in antioxidant defence is one of the proposed biological pathways that may contribute to the observed health benefits. The aim of our study was to determine the effect of moderate white wine consumption on the expression of GPx (glutathione peroxidase), CAT (catalase), NQO1 (NAD(P)H Quinone Oxidoreductase 1) and HSP-70 (heat shock protein) in cardiomyocytes. Materials and methods: Male Sprague Dawley rats were given either a combination of white wine (Graševina, Krauthaker, 13% alcohol vol.) and water (n= 6) or only water (n=7) for 28 days. After that, the animals were sacrificed. From the harvested hearts, cardiomyocytes were isolated, fixated and stored at -20 ⁰C. When all the samples were collected, cells were permeabilized, incubated with antibodies and analysed by flow cytometry. Results: Consumption of white wine increased HSP- 70 expression by 1.46 ± 0.29-fold (p=0.0002) when compared to control group. Furthermore, expressions of CAT, GPx and NQO1 were increased in wine consuming rats by 1.3 ± 0.21 (p=0.008), 1.45 ± 0.33 (p=0.0006), and 1.36 ± 0.12 (p=0.003) fold, respectively. Conclusion: Moderate white wine consumption for 4 weeks resulted in increased expression of the enzymes that are important in the cell protection from the oxidative stress.

white wine ; rat cardiomyocytes ; oxidative stress

nije evidentirano