engleski

Influence of particle size and sample – extracting mixture ratio on extraction of lutein from laying hens feed mixture

The role of lutein in human and animal health has already been investigated. Due to its importance, it is of great interest to determine its content in the food we consume or is consumed by animals whose products we use in our diet. In this study, the influence of particle size and dry matter (sample):extraction mixture ration on determination of lutein content in laying hens feed was investigated. Mixture of methanol and acetone, 0.2% BHT solution and 40% KOH solution was used as extraction mixture. All extractions were performed on the same samples and assisted by an ultrasonic probe. The lutein content of the samples was determined by means of HPLC with a UV- VIS detector at 450 nm. In ground samples, the determined content of lutein in mg / kg of sample is 52% better compared to ungrinded samples. Comparing the results obtained by the same method but with saponification, 3% more lutein was determined with saponification and the RSD value was significantly reduced (from 1.498% to 0.420%). The ratios of dry matter:extraction mixture: 1: 5, 1:10, 1:20 and 1:40 were investigated, and the obtained results (3, 152 ; 3, 956 ; 5, 148 and 2, 015 mg / kg of sample) indicate that the best ratio is 1:20.

lutein ; particle size ; ultrasound assisted extraction

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