engleski

Correlation of fecal calprotectin with salivary inflammatory markers

Introduction: Oral cavity is the initial part of the alimentary canal and saliva is an excellent medium for assessment of local immunity, inflammatory bowel disease (IBD) due to the abundance of pro-inflammatory markers, especially in dysbiosis. Microbiological and inflammatory changes originating from the gingival niche, might lead to a worsening of IBD via saliva. In turn, it is to be expected that IBD leads to an increase of pro-inflammatory cytokines in saliva. Clinically expressed or asymptomatic intestinal inflammation appears in more than 50% of patients with spondyloarthritis (SpA). The aim of this study was to analyze correlations between fecal calprotectin and inflammatory markers in saliva depending on C- reactive protein (CRP). Methods: In this prospective study we enrolled 184 patients with SpA of age from 18 to 80 years, diagnosed by rheumatologist according the Assessment of Spondyloarthritis International Society non- radiographic axial SpA criteria and signed the Informed Consent Form. Patients completed the Graded Chronic Pain Scale (GCPS) Questionnaire version 2.0 and the Patient Health Questionnaire (PHQ)-15: physical symptoms. Inflammatory markers were analyzed in stool (fecal calprotectin), unstimulated saliva (matrix metalloproteinase-3, IL-12/IL-23 p40, IL-17 and TNF-α), serum (CRP). Fecal calprotectin and CRP were analyzed by routine laboratory methods (automated analyzer) and inflammatory markers in saliva with ELISA. Results: Fecal calprotectin correlated statistically significantly with matrixmetalloproteinase-3 in the saliva of SpA patients (P=0.0005) and physical health symptoms (P=0.028), but not with the degree of chronic pain and the concentration of pro-inflammatory cytokines (TNF-a, IL-17, IL-12/IL-23 p40). Fecal calprotectin did not correlate with the disease onset and the duration of the disease, but it correlated with the IBD activity measured with CRP. Conclusion:Specific pro-inflammatory markers in saliva can reflect inflammatory conditions in the intestines.

calprotectin, fecal ; C-reactive protein ; inflammatory bowel disease ; metalloproteinase -3 ; saliva ; spondyloarthritis

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