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The role of MATH-BTB family proteins TaMAB2 and AtBPM1 in plant development and stress response

MATH-BTB proteins are substrate-specific adaptors of CUL3-based E3 ligases, promoting ubiquitination and proteasomal degradation of target proteins. In this work, MATH-BTB proteins of wheat Triticum aestivum (TaMAB) and Arabidopsis thaliana (AtBPM) were phylogenetically analyzed. Forty-six putative TaMAB genes were retrieved from EnsemblPlants database. The majority of TaMAB proteins clustered in the grasses-specific expanded clade of MATH-BTB proteins. AtBPM proteins characteristically clustered in the smaller core clade. Two members, wheat TaMAB2 and Arabidopsis AtBPM1, were functionally analyzed. Overexpression of TaMAB2 in Arabidopsis affected epidermal cell length, indicating involvement in cytoskeletal regulation. TaMAB2 colocalized with ubiquitin, indicating possible involvement in the Cul3-E3 ligase complex. To assess whether environmental conditions affect AtBPM genes and AtBPM1 protein, Arabidopsis wild type plants and transgenic plants overexpressing AtBPM1 were exposed to osmotic and salt stress, different levels of light exposure and elevated temperature. Downstream analyses revealed perturbations of AtBPM genes and AtBPM1 protein on a transcriptional, post-transcriptional and post-translational level in different experimental conditions, indicating complex environment-dependent regulation.

Arabidopsis, drought, heat stress, MATH-BTB, protein stability, wheat

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