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Pregled bibliografske jedinice broj: 1217208

Visualization and identification of metabolically active microbial populations in hot spring biofilm


Kostešić, Ema; Mitrović, Maja; Pjevac, Petra; Orlić, Sandi
Visualization and identification of metabolically active microbial populations in hot spring biofilm // 13TH INTERNATIONAL CONGRESS ON EXTREMOPHILES September 18/22, 2022 - Loutraki - GREECE
Loutráki, Grčka, 2022. str. 1-1 (predavanje, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1217208 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Visualization and identification of metabolically active microbial populations in hot spring biofilm

Autori
Kostešić, Ema ; Mitrović, Maja ; Pjevac, Petra ; Orlić, Sandi

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13TH INTERNATIONAL CONGRESS ON EXTREMOPHILES September 18/22, 2022 - Loutraki - GREECE / - , 2022, 1-1

Skup
13TH INTERNATIONAL CONGRESS ON EXTREMOPHILES

Mjesto i datum
Loutráki, Grčka, 18.09.2022. - 22.09.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hot spring, biofilm, 16S rRNA, Boncat, CARD-FISH

Sažetak
The composition and activity of select populations in microbial hot spring biofilms is strongly influenced by fluctuations in temperature and the availability of electron donors and acceptors. To gain further insights into these complex communities, we characterized the microbial communities by 16S rRNA gene amplicons sequencing, and identified active populations under different substrate amendments and incubation conditions. Bioorthogonal noncanonical amino acid tagging (BONCAT) was applied to reveal active cells by tracking the incorporation of synthetic amino acids into newly synthesized proteins, which are later detected by fluorescent staining using azide-alkyne click chemistry. Subsequently, the phylogenetic identity of translationally active cells was determined by combining BONCAT with catalyzed reporter deposition rRNA-targeted fluorescence in situ hybridization (CARD-FISH). Interestingly, the anabolic activity of Gammaproteobacteria and Chloroflexi was increased in almost all incubations regardless if organic or inorganic substrate was amended, but the highest activity was observed in glucose and thiosulfate amended incubations.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Biotehnologija, Interdisciplinarne biotehničke znanosti



POVEZANOST RADA


Projekti:
HRZZ-PZS-2019-02-7373 - Višefazni pristup za dešifriranje mikrobne ekologije i biotehnološkog potencijala geotermalnih izvora u Hrvatskoj (MAMEBio) (Orlić, Sandi, HRZZ - 2019-02) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Sandi Orlić (autor)

Avatar Url Ema Kostešić (autor)

Avatar Url Maja Mitrović (autor)


Citiraj ovu publikaciju:

Kostešić, Ema; Mitrović, Maja; Pjevac, Petra; Orlić, Sandi
Visualization and identification of metabolically active microbial populations in hot spring biofilm // 13TH INTERNATIONAL CONGRESS ON EXTREMOPHILES September 18/22, 2022 - Loutraki - GREECE
Loutráki, Grčka, 2022. str. 1-1 (predavanje, međunarodna recenzija, sažetak, znanstveni)
Kostešić, E., Mitrović, M., Pjevac, P. & Orlić, S. (2022) Visualization and identification of metabolically active microbial populations in hot spring biofilm. U: 13TH INTERNATIONAL CONGRESS ON EXTREMOPHILES September 18/22, 2022 - Loutraki - GREECE.
@article{article, author = {Koste\v{s}i\'{c}, Ema and Mitrovi\'{c}, Maja and Pjevac, Petra and Orli\'{c}, Sandi}, year = {2022}, pages = {1-1}, keywords = {hot spring, biofilm, 16S rRNA, Boncat, CARD-FISH}, title = {Visualization and identification of metabolically active microbial populations in hot spring biofilm}, keyword = {hot spring, biofilm, 16S rRNA, Boncat, CARD-FISH}, publisherplace = {Loutr\'{a}ki, Gr\v{c}ka} }
@article{article, author = {Koste\v{s}i\'{c}, Ema and Mitrovi\'{c}, Maja and Pjevac, Petra and Orli\'{c}, Sandi}, year = {2022}, pages = {1-1}, keywords = {hot spring, biofilm, 16S rRNA, Boncat, CARD-FISH}, title = {Visualization and identification of metabolically active microbial populations in hot spring biofilm}, keyword = {hot spring, biofilm, 16S rRNA, Boncat, CARD-FISH}, publisherplace = {Loutr\'{a}ki, Gr\v{c}ka} }




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