engleski

Effect of carnosine on vascular function of Sprague- Dawley rats on a high-salt diet – a pilot study

INTRODUCTION: Previously, we showed that increased NaCl dietary intake impairs vascular function via increased oxidative stress. Carnosine (CAR ; β- alanyl-L-histidine) is a dipeptide that has anti- inflammatory, antioxidant activity and acts as a "scavenger" of ROS, reduces the levels of lipid peroxidation and low-density lipoprotein in the serum of aged rats. However, there are no studies that have examined the effect of carnosine supplementation on vascular function and oxidative stress levels following high salt intake. AIM: Present pilot study aimed to investigate the effect of carnosine supplementation on vascular function by examining acetylcholine-induced dilation of isolated aortic rings. MATERIALS AND METHODS: Healthy Sprague-Dawley rats (8-10 weeks old) were divided in four groups (3 rats per group) – CTRL (control group, 0.4% NaCl), HS (high salt diet, 4% NaCl in diet for 7 days), CTRL+CAR (oral carnosine supplementation, 150 mg/kg/day for 7 days) and HS+CAR group (HS and carnosine supplementation). Response to ACh and SNP (sodium nitro-prusside) was studied in isolated norepinephrine precontracted aortic rings (Isolated Organ Bath (Experimetria LTD)). All experimental procedures conformed to the European Guidelines for the Care and Use of Laboratory Animals (directive 86/609) and were approved by the local and national Ethical Committees (#2158- 61-46-22-14, EP355/2022). RESULTS: These preliminary results showed impaired ACh-induced relaxation of aortic rings in HS group compared to CTRL, and restored relaxation after carnosine supplementation. There are no differences in response to SNP among tested groups. CONCLUSION: Results suggest positive effect of carnosine on vascular function of rats on high salt diet. ACKNOWLEDGMENT: This study was supported by European Structural and Investment Funds through a grant to the Croatian National Science Center of Excellence for Personalized Health Care, Josip Juraj Strossmayer University of Osijek # KK.01.1.1.01.0010. and by institutional grants from the Faculty of Medicine Osijek IP1-2022-MEFOS (PI Ines Drenjančević) and IP16-2022-MEFOS grant (PI Zrinka Mihaljević).

carnosine, high salt diet, oxidative stress, vascular function, aortic rings, Sprague-Dawley rats

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