engleski

Antiproliferative and anti-inflammatory activity of pregnane steroid isolated from the Adriatic Sea coral, Eunicella cavolini

Gorgonian corals of the genus Eunicella are well- known sources of structurally diverse secondary metabolites including terpenoids, steroids as well as alkaloids to lower extent. Detailed research studies of those compounds are scarce and they have been examined mostly as anticancer agents on several cell lines since [1]. Therefore, within the Adriatic Sea bioprospecting programme, we have carried out the extraction of the coral, Eunicella cavolini (Koch, 1887). Upon dereplication by using spectroscopic data and MarinLit database, we successfully isolated a pregnane steroid and connected it to previously isolated compound [2]. Furthermore, the isolated steroid was screened for antiproliferative activity against four cancer and one non-transformed cell line accompanied with evaluation of anti-inflammatory potential by using assays of albumin denaturation and soybean lipoxygenase inhibitions. The natural compound exhibited non-selective activity against tested cell lines with the highest activity towards hepatocellular carcinoma (HepG2) (IC50 = 6 µM). Finally, at the concentrations of 309.4 and 118.6 µM, pregnane steroid inhibited 50% of soybean lipoxygenase and albumin denaturation, respectively.

Eunicella cavolini, pregnane steroid, anticancer, anti-inflammatory

nije evidentirano