Comparative HILIC-UPLC analysis of immunoglobulin G N-glycome from saliva and plasma (CROSBI ID 723303)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Radovani, Barbara ; Lauc, Gordan ; Gudelj, Ivan
engleski
Comparative HILIC-UPLC analysis of immunoglobulin G N-glycome from saliva and plasma
Immunoglobulin G (IgG) is the most abundant antibody in the blood and plays a critical role in host immune defence against infectious pathogens. IgG glycosylation is known to modulate IgG effector functions and is involved in the development and progression of disease. Because the reactivity of salivary IgG mirrors that of plasma IgG, saliva is an attractive alternative to blood for studies in which antibody analysis is intended to provide an indication of a subject's immune status. Therefore, in this study, we described a rapid and effective method for comparative N-glycome analysis of IgG from human plasma and saliva samples. Plasma and saliva were collected from 9 healthy volunteers within a 2- hour time window. IgGs were affinity-purified from the two biofluids with protein G-agarose beads and then denatured and enzymatically deglycosylated. The released N-glycans were analysed by ultra-high performance liquid chromatography with hydrophilic interaction analysis (HILIC-UPLC). IgG from saliva exhibited reduced galactosylation and sialylation compared with IgG from plasma, whereas core fucosylation remained unchanged. The slightly proinflammatory profile of salivary IgG may be due to higher exposure to pathogens compared with plasma IgG. This study provides an additional tool for the analysis of salivary IgG glycosylation and highlights its potential as a surrogate biomarker for the study of plasma IgG.
N-glycosylation, immunoglobulin G, saliva, biomarker
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Podaci o prilogu
Poster 23
2022.
objavljeno
Podaci o matičnoj publikaciji
Joint Glycobiology Meeting 18-20 September 2022: Program with abstracts
Utrecht: Dutch Society for Glycobiology
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Podaci o skupu
32st Joint Glycobiology Meeting
poster
19.09.2022-20.09.2022
Utrecht, Nizozemska