The effect of pulse corticosteroid therapy on peripheral blood lymphocyte apoptosis in patients with relapsing-remitting multiple sclerosis - preliminary results (CROSBI ID 491309)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Petravić, Damir ; Zurak, Niko ; Petelin, Željka ; Mahović, Darija ; Golemović, Mirna ; Franjić, Jasna ; Ribarić, Branka
engleski
The effect of pulse corticosteroid therapy on peripheral blood lymphocyte apoptosis in patients with relapsing-remitting multiple sclerosis - preliminary results
The aim of the study was to assess the expression of CD95 molecule on peripheral blood (PB) CD4+ and CD8+ T-lymphocytes in patients with relapsing-remitting multiple sclerosis (RRMS) in the relapse of the disease, immediately before and after pulse corticosteroid therapy with regard to the presumed mechanism of corticosteroid effect on the induction of apoptosis of autoreactive peripheral blood lymphocytes. The study included 8 patients (5F, 3M) with RRMS diagnosed by McDonald's criteria. Mean age of patients was 34.88± 4.1 years and mean duration of the disease 3.5± 4.97 years. The analysis of T-cells was done in the relapse of the disease, immediately before and after pulse corticosteroid therapy consisting of intravenous methylprednisolone 1000 mg/day in 500 ml physiologic saline in short infusions over five days. The proportions of T-lymphocytes and CD95+ T-lymphocyte subsets were analysed by use of monoclonal antibodies (anti-CD4-PE/Cy5, anti-CD8-FITC and anti-CD95-PE, DAKO) and flow cytometry (FACSscan cytometer and CellQuest software, Becton Dickinson). On statistical analysis nonparametric Wilcoxon test for dependent samples was used. Results were presented as median and range. A p value <0.05 was considered statistically significant. The proportion of CD8+ T-lymphocytes was significantly higher immediately before pulse corticosteroid therapy (M=37.65% ; range 33.3%-48.5%) in comparison to the period after corticosteroid therapy (M=28.7% ; range 22.1%-41.5%, p=0.012). The CD4/CD8 ratio was significantly lower before (M=1.08 ; range 0.64-1.44), than after (M=1.44 ; range 0.8-2.45, p=0.017) pulse corticosteroid therapy. The proportion of CD8+ T-lymphocytes bearing CD95 was significantly higher immediately before (M=31.6% ; range 25.4%-39.1%), than after (M=20.45% ; range 15%-30%, p=0.012) pulse corticosteroid therapy. The proportions of CD4+ T-lymphocytes and CD4+CD95+ T-lymphocytes were not significantly different before and after pulse corticosteroid therapy. The change of CD95 expression on peripheral blood CD8+ T-lymphocytes shows that pulse corticosteroid therapy influences on apoptosis of autoreactive lymphocytes in patients with RRMS.
apoptosis; corticosteroid therapy; relapsing-remitting multiple sclerosis
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Podaci o prilogu
57-57.
2003.
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objavljeno
Podaci o matičnoj publikaciji
Neurologia Croatica. Supplement
1331-5196
Podaci o skupu
Dubrovnik International Conference on Multiple Sclerosis and Continuing Education (2 ; 2003)
poster
21.05.2003-24.05.2003
Dubrovnik, Hrvatska