engleski

RAD-Seq in plant phylogeography: case study of Festuca varia complex (Poaceae) on the Balkan peninsula

High-throughput, restriction site-associated DNA sequencing (RAD-seq) is widely used for research in fields of population genetics (e.g. for calculations of genetic diversity, distance and structure) and phylogenomics (integrative evolution and genomic analyses on next-generation sequencing data) which combined with spatial spans of investigated populations can provide valuable insights into their phylogeographic patterns. Originally RAD-seq was used to untangle complex infraspecific relationships (among populations of the same species), but nowadays is often used to elucidate interspecific relationships within recently derived phylogenetic groups. Here we present the overview of the original RAD-seq methodology implemented in our research with comprehensive subsequent bioinformatic analyses, pipelines and programs used. Important findings of different analysed short nucleotide polymorphisms (SNP) datasets are shown through most recent and comprehensive published articles dealing with plant species from various genera (e.g. Aurinia, Dianthus, Euphorbia and Helleborus), all conducted in south-eastern Europe. Additionally, our preliminary results from 17 sequenced populations within F. varia complex (Poaceae) from the Balkan and the Apennine Peninsulas and Alps are presented.

RAD-Seq ; phylogeography ; Festuca

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