engleski

The effect of Globularia alypum L. methanolic extract on seed germination and on the level of oxidative stress in Raphanus sativus L. seedlings

The aim of this study was to explore the effects of Globularia alypum L. methanolic (MeOH) extract on radish (Raphanus sativus L.) seed germination and on the level of non-enzymatic antioxidants in radish seedlings in the presence of excess copper ions (II). Seeds (25 per replicate) were pre- treated (pre-soaked) for two hours with G. alypum MeOH extract dissolved in distilled water (50, 500 and 5000 µg/mL) and afterwards treated with 3.5 mL of 0.005 M CuSO4. After five days of germination in light at 20 ± 2 °C, germination parameters were assessed, the root and hypocotyl lengths measured, and the dry masses of radish seedlings weighed. Afterwards, seedling tissue homogenates were prepared using 5% (w/v) trichloroacetic acid (1:10). The Folin-Ciocalteu’s reagent was used to determine the total phenolic contents, while glutathione (GSH) levels were evaluated using the Ellman’s reagent. All measurements were performed in triplicate. Significant differences between copper treatment and negative control (distilled water) were assessed using Student’s t test, and those between copper treatment with and without G. alypum pre-treatment using one way analysis of variance (ANOVA) with Dunnett’s post-hoc test (α = 0.05). Treatment with 0.005 M CuSO4 significantly decreased all measured parameters compared to negative control. The two lower concentrations of G. alypum MeOH extract (50 and 500 µg/mL) significantly increased germination percentages of radish seeds (76 ± 0 and 76 ± 11% vs. 52 ± 14%), while all extract concentrations increased hypocotyl lengths (5.60 ± 0.21, 5.91 ± 0.23 and 6.46 ± 0.03 mm vs. 4.90 ± 0.27 mm). Furthermore, all MeOH extract solutions significantly increased total phenolic content and GSH levels of radish seedlings. Obtained results indicate that G. alypum may reduce the negative effects of copper induced oxidative stress in radish seeds.

copper-induced oxidative stress ; germination parameters ; hypocotyl and radicle length

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