hrvatski

Korekcija broja eritroblasta i leukocita kod pacijentice s hemoglobin E-beta talasemijom – prikaz slučaja

Introduction: The aim of this study is to present a case of the correction of nucleated red blood cell (NRBC)and white blood cell (WBC) count in a 32-year-old woman of South Asian origin with Hemoglobin E–beta thalassemia, followed in University Hospital Dubrava. Case report: The patient was followed for 16 months during regular monthly examinations in hematology clinic where her blood was collected in K3-EDTA coated tubes. Samples were first analyzed by Advia 2120i(Advia), followed by WBC count by flow cytometry(FCM ; CD45-ECD) and NRBC count/500WBCs in peripheral blood (PB) smear. Results obtained were divided into two groups: 1) Advia NRBC count Advia linearity range (112-295%) and 2) Advia NRBC count close to/beyond linearity range (≥295%). Within the group we compared WBC count between FCM and Advia auto-corrected (FCM/Advia) and between FCM and WBC count obtained by mathematical correction of uncorrected WBC count from Advia with NRBC count from PB smear (FCM/calculated). Data is expressed as medians and ranges. Differences between groups were compared using non-parametric Mann-Whitney test (P< 0.05 was considered statistically significant). Results: In group 1 WBC’s median (ranges): FCM 11(6, 1-15, 9), Advia 12, 2 (9, 3-16, 5), calculated 10, 5 (9, 2-16, 8), there was no statistically significant difference (FCM/Advia P=0, 310 ; FCM/calculated P=0, 402). In group 2 WBC’s median (ranges): FCM 10, 7 (6, 6-15, 6), Advia 14, 6 (11, 5-19, 9), calculated 11, 2 (8, 9-14, 6) there was no statistically significant difference for FCM/calculated (P=0, 805), but with difference being statistically significant for FCM/Advia (P=0, 026). Conclusion: Advia has algoritams which give reliable number of NRBCs in linear measurement range with an automatic correction of WBC and differential count, but NRBC count is not reliable above linearity range. In this case it is necessary to confirm NRBC count in PB smear. If NRBC count from Advia is above linearity range, it is necessary to report NRBC and WBC differential counts from PB smear and according to NRBC count from PB smear correct the uncorrected WBC count from Advia.

Eritroblasti, leukociti, korekcija, beta talasemija

nije evidentirano