engleski

Effects of climate change and pollution on caddisflies: insights from a multiple stress experiment

Water pollution and climate change negatively affect freshwater ecosystems, impairing their normal functions and balance. Various pollutants co-occur and interact, changing the way aquatic insects cope with stress. Accordingly, the aim of the current study was to investigate the single and combined effects of emerging contaminants (ECs) ; pharmaceuticals (PhACs) and endocrine disrupting compounds (EDCs) and elevated water temperature on aquatic insects at the aquatic- terrestrial habitat linkage. A laboratory microcosm experiment was conducted with a simplified freshwater food web containing nonvascular macrophytes and Micropterna nycterobia, Trichoptera larvae feeding mainly as shredders. Sampling included initial and several consecutive collections including all life stages (larvae, pupae and adult stage). Analyses such as total protein content, total lipid content, non- target metabolome and lipidome profiling were conducted in order to evaluate the response of M. nycterobia to selected stressors at molecular level. Increased water temperature had an impact on phenology causing earlier emergence of adults and a decrease in lipid mass. Furthermore, treatments with ECs lowered the protein mass in larvae and pupae, irrespective of the water temperature. Multiple stressor effect was noticed in treatments with combined ECs and elevated water temperature as the highest decrease in total protein content in larvae and pupae, and total lipid content in adults. Metabolome was more affected by ECs, while increased temperature had a greater effect on lipidome.

Trichoptera ; Climate change ; Emerging contaminants ; Multiple stressors

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