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Pregled bibliografske jedinice broj: 1213746

BPM1 regulates RdDM-mediated DNA methylation via a cullin 3 independent mechanism


Jagić, Mateja; Vuk, Tamara; Škiljaica, Andreja; Markulin, Lucija; Vičić Bočkor, Vedrana; Tokić, Mirta; Miškec, Karlo; Razdorov, Genadij; Habazin, Siniša; Šoštar, Marko et al.
BPM1 regulates RdDM-mediated DNA methylation via a cullin 3 independent mechanism // Plant Cell Reports (2022) doi:10.1007/s00299-022-02911-9 (znanstveni, online first)


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Naslov
BPM1 regulates RdDM-mediated DNA methylation via a cullin 3 independent mechanism

Autori
Jagić, Mateja ; Vuk, Tamara ; Škiljaica, Andreja ; Markulin, Lucija ; Vičić Bočkor, Vedrana ; Tokić, Mirta ; Miškec, Karlo ; Razdorov, Genadij ; Habazin, Siniša ; Šoštar, Marko ; Weber, Igor ; Bauer, Nataša ; Leljak Levanić, Dunja

Vrsta, podvrsta
Radovi u časopisima, znanstveni

Izvornik
Plant Cell Reports (2022)

Status rada
Online first

Ključne riječi
MATH-BTB · DNA methylation · RdDM · DDR complex · Protein interactions · Arabidopsis thaliana

Sažetak
The best-known function of MATH-BTB proteins, including Arabidopsis BPM proteins, is their role as substrate-specic adaptors of CUL3-based E3 ligases in the ubiquitin-proteasome pathway. This paper reports a new CUL3-independent role of BPM1 in RNA-directed DNA methylation (RdDM). Using quantitative and qualitative Y2H, pull down, microscale ther- mophoresis and FRET-FLIM, we demonstrate that BPM1 interacts with DMS3 and RDM1, components of the chromatin remodeling DDR complex involved in the recruitment of the RdDM methylation machinery. All three proteins colocalized predominantly in the nucleus. The MATH domain, which specically binds proteins destined for degradation, was not essential for interactions with DMS3 and RDM1. In plants overexpressing BPM1, endogenous DMS3 protein levels were stable, indicating that BPM1 does not induce proteasomal degradation. In RDM1-overexpressing plants, RDM1 was not ubiquitinated. Together, these results suggest that BPM1 does not mediate the degradation of DMS3 and RDM1. Addition- ally, overexpression of BPM1 caused increased global methylation levels as well as CHH methylation in promoters of two RdDM-regulated genes, FWA and CML41. Overall, BPM1 seems to have a stimulating eect on RdDM activity, and this role appears to be unrelated to its known function as a Cul3-based E3 ligase adaptor.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-6229 - MATH-BTB proteini kao regulatori transkripcije i RNA posredovane metilacije DNA u biljnom razvitku (PHYTOMETHDEV) (Leljak-Levanić, Dunja, HRZZ - 2016-06) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
GENOS d.o.o.

Poveznice na cjeloviti tekst rada:

doi rdcu.be link.springer.com

Citiraj ovu publikaciju:

Jagić, Mateja; Vuk, Tamara; Škiljaica, Andreja; Markulin, Lucija; Vičić Bočkor, Vedrana; Tokić, Mirta; Miškec, Karlo; Razdorov, Genadij; Habazin, Siniša; Šoštar, Marko et al.
BPM1 regulates RdDM-mediated DNA methylation via a cullin 3 independent mechanism // Plant Cell Reports (2022) doi:10.1007/s00299-022-02911-9 (znanstveni, online first)
Jagić, M., Vuk, T., Škiljaica, A., Markulin, L., Vičić Bočkor, V., Tokić, M., Miškec, K., Razdorov, G., Habazin, S. & Šoštar, M. (2022) BPM1 regulates RdDM-mediated DNA methylation via a cullin 3 independent mechanism. Prihvaćen za objavljivanje u Plant Cell Reports. [Preprint] doi:10.1007/s00299-022-02911-9.
@unknown{unknown, author = {Jagi\'{c}, Mateja and Vuk, Tamara and \v{S}kiljaica, Andreja and Markulin, Lucija and Vi\v{c}i\'{c} Bo\v{c}kor, Vedrana and Toki\'{c}, Mirta and Mi\v{s}kec, Karlo and Razdorov, Genadij and Habazin, Sini\v{s}a and \v{S}o\v{s}tar, Marko and Weber, Igor and Bauer, Nata\v{s}a and Leljak Levani\'{c}, Dunja}, year = {2022}, DOI = {10.1007/s00299-022-02911-9}, keywords = {MATH-BTB · DNA methylation · RdDM · DDR complex · Protein interactions · Arabidopsis thaliana}, journal = {Plant Cell Reports}, doi = {10.1007/s00299-022-02911-9}, title = {BPM1 regulates RdDM-mediated DNA methylation via a cullin 3 independent mechanism}, keyword = {MATH-BTB · DNA methylation · RdDM · DDR complex · Protein interactions · Arabidopsis thaliana} }
@unknown{unknown, author = {Jagi\'{c}, Mateja and Vuk, Tamara and \v{S}kiljaica, Andreja and Markulin, Lucija and Vi\v{c}i\'{c} Bo\v{c}kor, Vedrana and Toki\'{c}, Mirta and Mi\v{s}kec, Karlo and Razdorov, Genadij and Habazin, Sini\v{s}a and \v{S}o\v{s}tar, Marko and Weber, Igor and Bauer, Nata\v{s}a and Leljak Levani\'{c}, Dunja}, year = {2022}, DOI = {10.1007/s00299-022-02911-9}, keywords = {MATH-BTB · DNA methylation · RdDM · DDR complex · Protein interactions · Arabidopsis thaliana}, journal = {Plant Cell Reports}, doi = {10.1007/s00299-022-02911-9}, title = {BPM1 regulates RdDM-mediated DNA methylation via a cullin 3 independent mechanism}, keyword = {MATH-BTB · DNA methylation · RdDM · DDR complex · Protein interactions · Arabidopsis thaliana} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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