Distinct longitudinal changes in immunoglobulin G N-glycosylation associate with therapy response in chronic inflammatory diseases (CROSBI ID 313781)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Štambuk, Jerko ; Vučković, Frano ; Habazin, Siniša ; Hanić, Maja ; Novokmet, Mislav ; Nikolaus, Susanna ; Tran, Florian ; Schreiber, Stefan ; Franke, Andre ; Rosenstiel, Philip ; Lauc, Gordan ; Aden, Konrad ; Pezer, Marija
engleski
Distinct longitudinal changes in immunoglobulin G N-glycosylation associate with therapy response in chronic inflammatory diseases
Immunosuppressants and biologicals are widely used therapeutics for various chronic inflammatory diseases (CID). To gain more detailed insight into their downstream effects, we examined their impact on serum immunoglobulin G (IgG) glycosylation. We analyzed IgG subclass-specific fragment crystallizable (Fc) N-glycosylation in patients suffering from various CID using the LC-MS approach. Firstly, we compared IgG Fc N- glycosylation between 128 CID patients and 204 healthy controls. Our results replicated previously observed CID-related decrease in IgG Fc galactosylation (adjusted p-value range 1.70 × 10−2–5.95 × 10−22) and sialylation (adjusted p- value range 1.85 × 10−2–1.71 × 10−18). Secondly, to assess changes in IgG Fc N-glycosylation associated with therapy and remission status, we compared 139 CID patients receiving either azathioprine, infliximab, or vedolizumab therapy. We observed an increase in IgG Fc galactosylation (adjusted p-value range 1.98 × 10−2–1.30 × 10−15) and sialylation (adjusted p-value range 3.28 × 10−6–4.34 × 10−18) during the treatment. Furthermore, patients who reached remission displayed increased Fc galactosylation levels (p- value range 2.25 × 10−2–5.44 × 10−3) in comparison to patients with active disease. In conclusion, the alterations in IgG Fc glycosylation and the fact these changes are even more pronounced in patients who achieved remission, suggest modulation of IgG inflammatory potential associated with CID therapy.
chronic inflammatory diseases ; inflammatory bowel disease ; IgG glycosylation ; response ; personalized medicine ; autoimmune diseases
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
23 (15)
2022.
8473
15
objavljeno
1422-0067
10.3390/ijms23158473
Trošak objave rada u otvorenom pristupu
Povezanost rada
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje), Farmacija