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BPC 157 related inhinhibition of cell growth in human melanoma cell line BPC 157- related inhibition of cell growth in human melanoma cell line

Melanoma therapy still remains without notable success of any pharmacological agent. Gastric pentadecapeptide BPC 157 is a peptide with various pharmacological effects on different organ systems including wound healing and inflammation. Its possible effect on cell growth and differentiation was not investigated on human cancer cell lines. Materials and Methods: Human melanoma cells were maintained in RPMI-1840 medium supplemented with 10% FBS and antibiotics. Cells were grown to confluency in humidified incubator (95% air atmosphere, 5%.CO2) at 37°C. Upon reaching confluency, cells were maintained in chemically defined medium (CDM). BPC157 was added in the culture medium in the various concentrations (2 pg. and 2ng, respectively) for a period of 48 hours. Cell morphology was observed daily under light microscope and the photomicrographs were taken. After harvesting cells were prepared in EtOH for flow cytometry analysis. Results: Microscopy did not reveal any changes upon treatment with BPC 157 after 48h comparing to controls. Flow cytometry showed 99% of cells (p<0.01) were in G0-G1 phase. Control cells had total S-phase fraction of 9.11%. However, upon introduction of BPC 157, in concentration of 2pg/ml, the percentage of cells in S-phase was decreased by 20% compared to controls. Moreover, the concentration of 2 ng/ml BPC 157 lowered total S-phase fraction by 55% comparing to controls and by 30% comparing to BPC 157 in picogram values (p<0.01). Conclusion: We assume that gastric pentadecapeptide BPC 157 could act as a inhibitory agent during G1-S transition state of cell cycle thereby preventing DNA synthesis and cell growth.

melanoma cell line; inhibition of cell growth; BPC 157

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