engleski

Tandem mass spectrometry in combination with ion mobility for characterization of cardiolipin molecular diversity

Cardiolipin (CL) is a unique class of membrane phospholipid distributed almost exclusively in the mitochondrial inner membrane of eukaryotic cells. Extensive remodeling of CL occurs via a highly- conserved deacylation – transacylation process, and deficiency of the CL transacylase leads to Barth syndrome. Due to their unique structure, comprehensive characterization of CL molecular diversity is analytically challenging. Coupling traveling wave ion mobility and high-resolution tandem mass spectrometry (IM-MS/MS) enables a simple and rapid multidimensional characterization CL molecular diversity. Using IM-MS/MS we confirm that Saccharomyces cerevisiae mutants completely devoid of CL remodeling (taz1Δcld1Δ) exhibit a shift towards CL species with more saturated and shorter acyl chains. We further demonstrate that CL acyl chain composition of WT and taz1Δcld1Δ cells is strongly influenced by the presence of exogenously supplemented fatty acids in the culture media. IM-MS/MS provides a powerful tool for the structural identification of CLs in complex biological samples.

Cardiolipin ; Barth syndrom ; Ion mobility mass spectrometry

nije evidentirano