engleski

Employment of response surface methodology in optimizing microwave-assisted extraction of bioactive compounds from Teucrium montanum and Glechoma hederacea

Bioactive diversity and the economic accessibility of plant species have been the main factors for their integration into human diet and lifestyle since ancient times. In the last decade, the popularization of a healthy lifestyle along with increased consumption of phytochemicals from sources of natural origin has notably supported the trend of developing functional food products with the aim of preventing various health diseases. Arising from the presence of polyphenols as noteworthy carriers of bioactive potential, T. montanum and G.hederacea from the Lamiaceae family represent insufficiently explored aromatic plants. In the scope of integrating sustainable and “clean” solutions in food processing, multiple response modeling of microwave- assisted extraction (MAE) of bioactive compounds from T. montanum and G.hederacea was conducted. In order to determine the optimal extraction conditions, independent variables- temperature (50, 70 and 90 °C), time (3, 6 and 9 min) and solid-to- solvent ratio (1:100, 1:40 and 1:25 g/mL) were defined. The influence of process parameters was monitored by response factors, i.e. total phenolic content (TPC) and antioxidant capacity applying ABTS (2, 2′-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay. Correlation between predicted and experimental values, as well as, the fit of statistical parameters for both plant species implied the reliability of each proposed regression model. Hence, a significant dependence of temperature and, both temperature and the solid-to-solvent ratio, was determined on the final extraction yield for T.montanum and G.hederacea, respectively. For achieving maximum TPC and antioxidant capacity, optimum conditions and predicted results were defined as follows: 70 °C, 6.00 min, 1:40 g/mL for T.montanum (TPC: 696.34 mg GAE/L, ABTS: 3.18 mmol TroloxE/L) and 90 °C, 8.84, 1:25 g/mL for G.hederacea (TPC: 1468.37 mg GAE/L, ABTS: 7.43 mmol TroloxE/L). This approach to extraction modeling has proven to be effective for defining the significance of process variables and their interactions to maximize extraction yield and cost- effectiveness.

Teucrium montanum ; Glechoma hederacea ; Microwave-assisted extraction ; Bioactive content ; Response surface methodology

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