engleski

Analysis of HPV infected cells using different biomarkers by mass cytometry

Cervical cancer is one of the most common malignancies among women despite existing prevention options. The development of cervical cancer is preceded by precancerous changes called cervical intraepithelial neoplasia (CIN) for several years. CIN is divided into three stages: CIN1 (mild dysplasia), CIN2 (moderate dysplasia), and CIN3 (severe dysplasia). Cervical cancer and CIN are associated with high-risk human papillomavirus (HR HPV) infection that is sexually transmitted and responsible more than 95% of cervical cancer cases among women. A biomarker is a characteristic that can be objectively measured and evaluated as an indicator of normal biological processes, pathogenic processes or pharmacological responses to a therapeutic intervention. HPV and cancer biomarkers may be helpful with cervical cancer diagnosis, potential therapy, predictive value and most important to help with preventive screening tests that are avalaible now. In this study mass cytometry was used which enables parallel identification of markers on individual cells by combination of flow cytometry and mass spectrometry with heavy metal isotopes. It allows simultaneous analysis of up to 40 different biomarkers and also barcoding of samples. LBC (Liquid based cytology) fixed cell lines and HPV infected cells were assessed for the presence of several markers by mass cytometry. Further plans are to assess more cervical samples and biomarkers and include the information collected through a 2- year follow up period to find those associated with disease or virus persistence or progression. Key words: biomarker ; cervical cancer ; HPV ; mass cytometry

biomarker ; cervical cancer ; HPV ; mass cytometry

nije evidentirano