engleski

Hematological parameters in broiler breeder flock fed diet supplemented with natural 1,25(OH)2D3- glycosides

Vitamin D is an important factor in calcium metabolism, bone development and eggshell formation in laying hens. However, it also has an important function in improving immune response and lowering inflammatory reaction acting on several signaling pathways. In this field trial we monitored the effect of natural 1, 25(OH)2D3-glycosides from waxy-leaf night shade (Solanum glaucophyllum) on hematological parameters of broiler breeder hens from 55 till 61 weeks of age. Two separate houses with 5000 animals of Ross 308 breeders each received standard feed (CON) or supplemented with 1 µg of 1, 25(OH)2D3-equivalents (SG), with total vitamin D content in both treatments at 3200 IU. Blood samples were taken at 14, 25 and 35 days after the beginning of trial and tested for number of red and white blood cells, hematocrit and hemoglobin concentration. Gained data were compared between treatments per time point and within treatment over time using LSD-model with significance set on p ≤ 0.05. Level of red blood cells move between 2, 32 ± 0, 21 and 2, 7 ± 0, 42 x1012/L, with rise till 25 days after start of treatment in both groups, with slight drop till 35 days in both groups, without significant differences between groups. Number of white blood cells in SG group significantly dropped at 35 days of age from 12, 64 ± 5, 42 to 3, 74 ± 1, 68 x109/L, and was significantly lower compared to all sampling days within the group, as well as compared to CON group which rose to 16, 04 ± 4, 37 x109/L at 35 days. Hematocrit values in group SG rose significantly at 35 days within the group to 0, 35 ± 0, 033 L/L, without significant difference between treatments. Hemoglobin concentration rose continuously in SG group until the end of the trial, but with significantly higher values in CON group 25 days after start, at 90, 94 ± 11, 47 g/L, both between the treatments and among sampling times. Results showed continuous rise and persistence of red blood cell number, hematocrit and hemoglobin concentration parameters in the group with added natural vitamin D. However, number of white blood cells until the end of trial significantly dropped what could indicate modulation of immune and inflammatory reactions in the organism.

Solanum glaucophyllum ; vitamin D metabolism ; hematology ; broiler breeders

nije evidentirano