engleski

In vitro generation of embrioid bodies from mouse embryonic stem cells: potentially source of insulin-secreting structures

Stem cells are self-renewing elements that can generate the many cell types in the body. These cells are derived from an early stage of the embryo and are named embryonic stem (ES) cells. ES cells isolated from 129/sv mice can be differentiate into insulin-producing cells. Because of numerous patients with Diabetes mellitus, transplantation of in vitro produced Langerhans islets causes successful protection from development of diabetic late complication. When injected into diabetic mice, these cells undergo rapid vascularization and maintain a clustered, islet-like organization. There is no data about such work on NOD (non-obesity) mice, which spontaneously developed Diabetes mellitus. The aim of our work was generated structure like this from blastocysts of NOD mice (project no. 098098). The procedure involved some steps and until today we successful generated embryoid bodies, which presents the base levels for expansion of insulin-producing cells. Blastocysts are isolated from uterus of 3.5 days pregnant NOD mice. After 48 hours of culturing the zona pellucida was dissolved in acid media. Inner cell mass of blastocysts was expanded on gelatin-coated tissue culture surface in medium for ES cells in the presence of leukemia inhibitory factor. Four days later, in medium for embryonic cells without LIF were generated embryonic bodies. In the future work, these cells should be propagated in insulin secretion cells by using specific condition. This is a good way for producing of immunocompatible tissue for transplantation in diabetic recipients.

stem cells; islet; diabetes mellitus

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