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Frequency of micronucleated erythrocytes in the rat bone marrow exposed to 2.45 GHz radiation

In vivo mammalian cytogenetic test, erythrocyte micronucleus assay was used to investigate the extent of genetic damage in the bone marrow red cells of rats exposed to RF/MW irradiation. Wistar rats (N=40) were exposed to 2.45 GHz continuous, radiofrequency microwave (RF/MW) field 2 hours daily, 7 days weekly, at power density 5-10 mW/cm2. Four subgroups were created in order to be irradiated 4, 16, 30 and 60 hours. Sham-exposed controls (N=40) were included in the study. Animals were euthanized on final irradiation day of each treated subgroup1). Bone marrow smears were examined to determine the extent of genotoxicity after particular treatment time. Mann-Whitney test (p<0.05) was used for statistical evaluation of data. After 15 irradiation treatments of two hour each, the frequency of micronucleated bone marrow red cells was significantly increased in comparison to sham - exposed subgroup. Pairwise comparison of data obtained after 2, 8 and 30 irradiation treatments did not reveal statistically significant differences between sham and treated subgroups (Figure 1). It might be concluded that under the applied experimental conditions, RF/MW irradiation initiates transitory cytogenetic effect manifested with micronucleus formation in erythropoietic cells.

erythrocyte micronucleus assay; RF/MW irradiation; erythropoietic cells

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