engleski

The acute effects of pegylated single-walled carbon nanotubes on the protein oxidative damage and HSP-70 levels in primary mouse astrocytes exposed to stretch injury

Aims: We have previously shown that the application of single-walled carbon nanotubes chemically functionalized with polyethylene glycol (PEG-SWCNTs) influences the expression of some proteins and causes changes in the cytokine release from the cultured astrocytes exposed to severe in vitro traumatic brain injury (TBI). Still, questions remain regarding the potential toxicity of the investigated nanomaterial. Here, we tested the effects of PEG-SWCNTs on oxidative protein damage and the expression of a stress protein, heat shock protein 70 (HSP-70). Methods: Primary mouse astrocytes were grown on 6- well plates with deformable membranes and subjected to severe stretch injury. PEG-SWCNTs or vehicle were applied at 1 h post-injury and the astrocytes were collected 23 h later. The dot blot method was used to detect the levels of oxidatively damaged proteins, and the Western blot was used to determine the expression levels of HSP-70. Non-injured, vehicle-treated astrocytes were used as the control group. Results: Stretch injury caused a slight, but non- significant increase in the levels of oxidatively damaged proteins and the application of the PEG- SWCNTs to the injured astrocytes did not cause additional protein oxidation. In vitro trauma, with or without PEG-SWCNTs treatment, had no effect on the HSP-70 levels in astrocytes. Conclusions: Results of this study suggest that PEG-SWCNTs do not cause oxidative protein damage in astrocytes exposed to in vitro TBI, nor do they increase the level of cell stress as determined by measuring the HSP-70 levels. This research was fully supported by the Croatian Science Foundation grant UIP-2017-05-9517 to KP.

astrocytes ; heat-shock protein 70 ; nanotubes, carbon ; mouse ; oxidative Stress ; traumatic brain injury

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