engleski

Preliminary histological comparation of injectable biphasic calcium phosphate and anorganic bovine bone in alveolar bone regeneration

Background and Aim: Atrophy of the alveolar ridge lead to damage of the bone structure. Nowadays, many different biomaterials are used to reduce dimensional changes. The aim of this study was to examine the regenerative potential and measure the percentage of newly formed bone (NB), residual biomaterial and connective tissue by histological examination of injectable biphasic calcium phosphate (I-BCP) composed of 60% Hydroxyapatite (HA) and 40% Beta – tricalcium phosphate (β- TCP).The hypotheses are that injectable biphasic calcium phosphate (I-BCP) achieve approximately the same percent effect of NB compared to anorganic bovine bone (ABB) and that I-BCP will have the same histocompatibility compared to ABB. Methods: Thirty-six subjects participated in the study and were divided into two groups. After extraction of the hopeless tooth, the socket in the studied group is filled with I-BCP, while the socket in the control group is filled with ABB. After 6 months of healing, a biopsy is taken. Pathohistological analysis evaluated the tissue response, while the percentage of NB, residual biomaterial and soft tissue was determined histomorphometrically. Results: Preliminary results of three samples of I-BCP showed that NB contained osteocytes, while osteoblasts were found at the boundary between the NB and the remaining I-BCP, indicating bone regeneration. There was no signs of an inflammatory reaction, indicating that I-BCP is histocompatible. Histomorphometric examination revealed 58.06%, 44.85%, and 39% NB ; 1.80%, 10.97%, 14.19% residual biomaterial and 40.14%, 44.17%, 46.81% soft tissue. Conclusions: Based on the preliminary results, we can conclude that I-BCP has good biocompatibility with the surrounding tissue. In addition, the injectable form of the biomaterial allows easier insertion into a three-dimensional defect. Further studies based on histological and histomorphological analysis of the injectable form are needed to draw more concrete conclusions about this type of biomaterial and its role in bone regeneration.

bone regeneration ; biomaterials ; histology ; histomorphometry

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