engleski

The influence of light on translational activity of hot spring biofilm

Background: Methods for monitoring microbial activity in an environmental context are key to answering one of the most important questions in microbial ecology, namely how microorganisms respond to environmental stimuli. Bioorthogonal non-canonical amino acid tagging (BONCAT) is a technique that can be used to tag the proteomes of a variety of phylogenetically diverse microorganisms from different habitats, including hot springs. In these extreme environments, pressure and the presence of light, along with high temperature, are the most important physical parameters affecting microbial communities. Objectives: The aim of this study was to determine the effect of light on the number of anabolically active microorganisms in the biofilm sample from the high temperature source Bizovacke toplice. Methods: The biofilm was incubated with the addition of L-homopropargylglycine (HPG) for 2 days in parallel with the presence and absence of light. After 24 and 48 hours, Cu(I)-catalysed azide-alkyne cycloaddition was performed to detect metabolically active cells. The sample was examined with an inverted microscope and cells were counted both manually and with daime software. Results: The presence of light decreased the number of anabolically active cells compared to the sample incubated in the dark. As for the time of incubation, the longer incubation period resulted in higher translational activity.

hot spring ; biofilm ; 16S rRNA ; Boncat

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