engleski

The antimicrobial effect of two different diodelaser photo-activated disinfection treatments on adental implant mixed biofilm: An experimental study

Background & Aim: Antimicrobial photo-activated disinfection treat-ment (PDT) was introduced as a potential peri-implantitis treatmentoption. The aim of this study was to evaluate the antimicrobial effectof novel 445 nm diode laser light (SiroLase Blue ; Dentsply Sirona ; Germany) and 0.1% riboflavin solution used as a photosensiting dyeon an implant biofilm and compare it with other more commonly used660 nm diode laser light PAD with methylene blue dye.Methods: The in vitro study was conducted on 40 titanium dentalimplants contaminated with a Staphylococcus aureus (SA) and Can-dida albicans (CA) solution. A suspension was prepared from microor-ganisms mixed together in the equal volume share. Implants werekept in the microbial solution for 21 days in aerobic conditions. Bio-film creation is confirmed via SEM microscopy.Implants were randomly divided into 4 groups: negative control (NC) -without surface treatment ; positive control (PC) treated with a 0.2%chlorhexidine- based solution ; PDT1–660 nm with methylene bluedye ; PDT2–445 nm with 0.1% riboflavin dye.Samples were collected with sterile plastic inoculating loops betweensecond and fifth implant thread and immersed in 0.5 ml of sterileBrain Heart Infusion. Pour plate technique was used to determinateviable cells count. After incubation period of 48 h at 37C CFU werecounted.Results: PDT1 and PDT2 group showed greater reduction of SA andCA compared to NC group, and no significant difference compared toPC group. PDT2 showed greater reduction of CA compared to PDT1group (p=0.04).Conclusions: A novel antimicrobial treatment with 445 nm diode laserlight with 0.1% riboflavin solution showed greater efficiency on CAreduction on implant surface biofilm compared to more commonlyused PAD treatment 660 nm diode laser light with methylene bluedye. Both PAD treatment showed SA and CA reduction comparableto CHX treatment

sealing ; gap ; microbiology ; dental implants ; laser

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