engleski

Microwave-Assisted Extraction of Polysaccharides from Brown Algae Cystoseira Compressa

Brown algae Cystoseira compressa is a source of numerous bioactive molecules including sulfated polysaccharide fucoidan. Due to its anticoagulant, anti-inflammatory and antiviral activities, fucoidan has a potential application in functional foods, cosmeceutical and pharmaceutical products. Fucoidan extraction is conventionally performed in hot aquatic or acidic solutions at high temperatures for several hours. To reduce extraction time, energy and solvent usage advanced extraction techniques such as microwave (MAE) and ultrasound (UAE) assisted extraction are applied. The aim of this paper was to optimize MAE parameters (solvent, solvent to sample ratio, temperature and time) in order to achieve the highest possible polysaccharide yield from Cystoseira compressa and to compare it with conventional extraction. First, the effect of different solvents (H2O, 0.1M HCl, 0.2M HCl, 0.1M H2SO4, 0.2M H2SO4) and solvent to sample ratios (15:1 and 30:1) on polysaccharide yield were tested. By using 0.2M H2SO4, the obtained yield was almost 5 times higher than with H2O and 30:1 solvent to sample ratio resulted in higher yield than 15:1 ratio. Resulting optimal parameters (0.2M H2SO4 and 30:1) were then used to optimize time (5, 10 and 20 min) and temperature (40, 60, 80 and 100 ℃) of MAE. Polysaccharide yield increased with temperature increase up to 100 ℃ while there was no statistical difference (p ≥ 0.05) between 5 and 20 min. Even though conventional extraction resulted in higher yield (24.47%) then MAE (16.19%), time was reduced from 3 h to 5 min what presents a significant advantage of MAE.

seaweed ; polysaccharides ; microwave-assisted extraction

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