engleski

Morphology changes in bacterial isolate from pharmaceutical wastewater under the effect of macrolide antibiotic

Of the large quantities of pharmaceuticals that enter the environment, antibiotics that act on microbial populations are the most common. Bacteria can develop the ability to biodegrade xenobiotics as a homeostatic response to the stress to which they are exposed by a particular compound. The acquisition of antibiotic resistance can lead to specific changes in bacterial metabolism or morphology that may be useful for bacterial survival under certain environmental conditions, e.g. in industrial effluents. Pharmaceutical wastewater may contain antibiotics as a group of active pharmaceutical ingredients. Therefore, autochthonous microorganisms found in such complex environments are considered to have adapted enzymatic potential. Furthermore, Morphological changes in bacterial cells may also be an indicator of the antibacterial mechanism of action. In this study, the effect of various erythromycin concentrations on the morphology of the autochthonous bacterial isolate Pseudomonas putida from pharmaceutical industrial wastewater was investigated. The experiments were performed under batch conditions. The changes in cell size and shape were determined by microscopic analysis to investigate the effect of erythromycin on bacterial growth. Microscopic analysis showed that filamentation of cells occurred at an erythromycin concentration of 200 mg/L, with cell length ranging from 6.86 to 19.57 μm during the exponential phase up to 36 hours. The autochthonous bacterial isolate has the ability to survive high concentrations of the macrolide antibiotic erythromycin, which manifests itself in cell morphological changes.

bacterial isolate ; macrolide antibiotic ; pharmaceutical wastewater

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