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Evaluation of the BD Stem Cell Enumeration Kit on the BD FACSCanto II flow cytometer using BD Facscanto clinical and BD facs diva software (CROSBI ID 311497)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Rimac, Vladimira ; Bojanić, Ines ; Gojčeta, Koraljka ; Golubić Ćepulić, Branka Evaluation of the BD Stem Cell Enumeration Kit on the BD FACSCanto II flow cytometer using BD Facscanto clinical and BD facs diva software // International journal of laboratory hematology, 43 (2020), 1; 61-67. doi: 10.1111/ijlh.13315

Podaci o odgovornosti

Rimac, Vladimira ; Bojanić, Ines ; Gojčeta, Koraljka ; Golubić Ćepulić, Branka

engleski

Evaluation of the BD Stem Cell Enumeration Kit on the BD FACSCanto II flow cytometer using BD Facscanto clinical and BD facs diva software

ntroduction: CD34+ hematopoietic stem cell (HSC) enumeration by cell flow cytometry is routinely used in clinical laboratories for monitoring of HSC mobilization into peripheral blood and assessment of the quality of HSC products. The modified ISHAGE protocol is the most often used procedure for determination of CD34+ cells using flow cytometry. The aim of this study was to evaluate BD Enumeration stem cell kit on flow cytometer BD facscanto II, using facscanto clinical and facsdiva softwares. Methods: Validation study included determination of within-run and between-run precision, trueness (bias), comparison of the test results analyzed on facscanto clinical and facsdiva softwares, assessment of linearity, specimen stability, and carryover. Results: For between-run precision, coefficients of variation (CVs) were all <10%, except for low control level on facsdiva software. CVs for within-run precision were <10%, except for high absolute count of CD34+ cells on facsdiva software. Comparison of data showed no statistically significant differences between facscanto clinical and facsdiva software (Spearman's rank correlation coefficients were .993 for % of CD34+ cells and 0.983 for absolute count of CD34+ cells). In linearity study, bias for all dilutions was < 20%, and carryover assessment cannot be considered significant on both softwares. There was a statistically significant difference (P = .044) in absolute count of CD34+ cells after 24 hours of storage, when using facscanto clinical software. Conclusion: BD Stem Cell Enumeration Kit can be used in routine laboratory work on BD FACSCanto II instrument, whereas facscanto clinical and facsdiva software were used for acquisition and data analysis.

CD34 ; flow cytometry ; hematopoietic stem cells ; transplantation ; validation study

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Podaci o izdanju

43 (1)

2020.

61-67

objavljeno

1751-5521

10.1111/ijlh.13315

Povezanost rada

nije evidentirano

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