engleski

IL-6 and IL-8 gene expression in male children with obesity: A possibly early atherosclerotic inflammatory predictor

Objectives: Atherosclerosis is one well know complication that can occur during obesity and it might have inflammation processes as important part of its pathology. In some previous study results show that interleukin 8 (IL-8) and interleukin 6 (IL-6) are associated with numerous different inflammatory processes in obese state and contribute to pathogenesis of atherosclerosis and other cardiovascular diseases (CVD). Aim of this study was to analyze if there is a correlation between IL-6 and IL-8 gene expression and other anthropometric and biochemical parameters in subcutaneous adipose tissue (SAT) of healthy male children as an early sign of metabolic dysregulation that can be connected with obesity in early childhood and have impact on later development of atherosclerosis. Participants and Methods: We determined changes in gene expression of IL-6 and IL-8 in SAT in lean and overweight/obese healthy male children. Tissue samples from SAT fat depots were obtained during surgery from 32 normal weight male children age 5, 12±3, 21 years, and 22 overweight/obese male children age 5, 95±3, 24 years, who underwent elective abdominal surgery having hernia repairs or orchidopexies at the Department for Pediatric Surgery of the University Hospital Osijek. Subject were divided in two groups by their BMI Z-score. On SAT samples immunohistochemistry for detection of CD163+ cells was performed and gene expression of IL-6 and IL-8 by quantitative RT-PCR with reference genes for data normalization was measured. Results: Children in the overweight/obese group showed a higher expression of IL-6 (p=0, 019) and IL-8 (p<0, 001) in subcutaneous adipose tissue compared to normal weight children. The expression of IL-6 in SAT correlated positively with the number of CD163+ cells in same adipose tissue compartment, as has the expression of IL-8. The expression of IL-8 in SAT also correlated positively with BMI Z- score, triglyceride serum concentration, average surface area of SAT adipocytes and gene expression of COL63. Conclusion: Increased gene expression of IL-6 and IL-8 in subcutaneous adipose tissue of male children could indicate a possibility that obesity and chronic low-grade inflammatory processes in subcutaneous adipose tissue during early childhood can contribute to pathogenesis of atherosclerosis in adulthood.

gene expressions, obesity, male children, atherosclerosis

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