engleski

The connection between leptin receptor gene expression, serum leptin concentration and body mass index in different malignant breast tumors depending on lymph node metastases presence

Goal: Previously we showed (Koprivić et al. Acta clinica Croatica*) that obese females have a significantly higher level of leptin, regardless of the malignant breast tumor type. Observed through body mass index, the most significant differences in serum leptin levels are in the luminal B1 group. This study aimed to assess whether there was an association between leptin receptor gene (LEPR) expression and serum leptin concentration with body mass index (BMI), depending on the presence/absence of lymph node metastases. Material and methods: 53 females with malignant breast cancer were divided into two groups depending on the presence or absence of lymph node metastases (21 negatives and 32 positive lymph nodes). According to the St. Gallen Conference on breast cancer, carcinomas are divided into 5 subgroups according to molecular analysis and related to the expression profile of certain genes: Luminal A, Luminal B HER2 negative (LUM B1), Luminal B HER2 positive (LUM B2), HER2 positive and triple-negative subgroup. This division is based on estrogen, progesterone, and the expression of human epithelial growth factor. The positive or negative lymph node was specified with the tumor-node metastasis system classification AJCC (American Joint Committee on Cancer). LEPR gene expression level was determined by quantitative real-time PCR and serum leptin concentration by ELISA method. Each female was also measured BMI. Correlation analysis was calculated using Pearson's correlation coefficient at 95% confidence of interval (SigmaPlot v11.2, USA). Results: Statistically significant negative correlation was found between serum leptin level and gene LPRT expression (r=-0.451, P=0.0403) and significant positive correlation between BMI and serum leptin level (r=0.771, P=0.0000425) in group of females with absence lymph node metastases. In female malignant breast tumor groups with presence of lymph node metastases only significantly positive correlation was observed between BMI and serum leptin level ((r=0.677, P=0.0000207). Conclusion: The association between the LPRT gene and serum leptin levels is more pronounced in malignant breast tumors with the absence of lymph node metastases. Regardless of the presence/absence of lymph node metastases, changes in serum leptin concentration depend on a woman's BMI status.

breast tumor, leptin, leptin receptor, BMI, lymph node

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