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Pregled bibliografske jedinice broj: 1202707

The Role of S-Layer Proteins in Probiotic Properties of Levilactobacillus brevis Strains Isolated from Human Milk Microbiota


Butorac, Katarina; Novak, Jasna; Leboš Pavunc, Andreja; Banić, Martina; Čuljak, Nina; Kazazić, Saša; Kazazić, Snježana; Šušković, Jagoda; Kos, Blaženka
The Role of S-Layer Proteins in Probiotic Properties of Levilactobacillus brevis Strains Isolated from Human Milk Microbiota // Book of Abstracts from "7th Croatian Congress of Microbiology with International Participation" / Sviličić Petrić, Ines ; Leboš Pavunc, Andreja ; Šantić, Marina ; Kifer, Domagoj (ur.).
Zagreb: Recedo digital j.d.o.o., 2022. str. 111-111 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1202707 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The Role of S-Layer Proteins in Probiotic Properties of Levilactobacillus brevis Strains Isolated from Human Milk Microbiota

Autori
Butorac, Katarina ; Novak, Jasna ; Leboš Pavunc, Andreja ; Banić, Martina ; Čuljak, Nina ; Kazazić, Saša ; Kazazić, Snježana ; Šušković, Jagoda ; Kos, Blaženka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts from "7th Croatian Congress of Microbiology with International Participation" / Sviličić Petrić, Ines ; Leboš Pavunc, Andreja ; Šantić, Marina ; Kifer, Domagoj - Zagreb : Recedo digital j.d.o.o., 2022, 111-111

ISBN
978-953-7778-18-7

Skup
7th Croatian Congress of Microbiology with International Participation

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 24.05.2022. - 27.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
breast milk microbiota ; Levilactobacillus brevis ; S-proteins

Sažetak
Infant’s intestinal microbiome is believed to be highly influenced by their own mother’s breast milk microbiota. Mother’s milk is a rich source of nutrients, bioactive components, but also a diversity of microorganisms that are important for the health of a newborn. The focus of our research was screening of the Lactobacillus strains that rarely, but preferably, express S-proteins. Among over 100 strains, we have identified S-proteins solely in the 4 strains of Levilactobacillus spp. This is the first report that describes S-proteins of Levilactobacillus brevis strains isolated from mother’s milk. Four L. brevis strains, MB1, MB2, MB13 and MB20, were identified by whole genome sequencing. Gene annotation confirmed the presence of S-layer proteins, which were further identified by SDS-PAGE coupled to LC-MS/MS. The examined L. brevis strains possess S-proteins of 40-50 kDa. Further characterization revealed that S-proteins protect bacterial cells against adverse environmental stresses, such as those in gastrointestinal tract (GIT) or during freeze- drying, but also interact during cell adhesion to intestinal surfaces. This was observed through increased survival of the L. brevis strains in simulated GIT conditions, increased adhesion to Caco-2 cell line and stronger inhibition of adhesion of pathogenic bacterium Escherichia coli 3014 to Caco-2 cell line, as mentioned properties were negatively affected after the removal of S- layer proteins from the cell surface. Results imply that S-proteins mediate functional properties of the producer strains. Overall, S- layer proteins of investigated L. brevis strains have the potential to act as key mediators for their functionalities.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekti:
IP-2019-04-2237 - Potencijalne terapijske biomolekule druge generacije probiotika (PRO-BIO 2.0) (Kos, Blaženka, HRZZ - 2019-04) ( CroRIS)


Citiraj ovu publikaciju:

Butorac, Katarina; Novak, Jasna; Leboš Pavunc, Andreja; Banić, Martina; Čuljak, Nina; Kazazić, Saša; Kazazić, Snježana; Šušković, Jagoda; Kos, Blaženka
The Role of S-Layer Proteins in Probiotic Properties of Levilactobacillus brevis Strains Isolated from Human Milk Microbiota // Book of Abstracts from "7th Croatian Congress of Microbiology with International Participation" / Sviličić Petrić, Ines ; Leboš Pavunc, Andreja ; Šantić, Marina ; Kifer, Domagoj (ur.).
Zagreb: Recedo digital j.d.o.o., 2022. str. 111-111 (poster, međunarodna recenzija, sažetak, znanstveni)
Butorac, K., Novak, J., Leboš Pavunc, A., Banić, M., Čuljak, N., Kazazić, S., Kazazić, S., Šušković, J. & Kos, B. (2022) The Role of S-Layer Proteins in Probiotic Properties of Levilactobacillus brevis Strains Isolated from Human Milk Microbiota. U: Sviličić Petrić, I., Leboš Pavunc, A., Šantić, M. & Kifer, D. (ur.)Book of Abstracts from "7th Croatian Congress of Microbiology with International Participation".
@article{article, author = {Butorac, Katarina and Novak, Jasna and Lebo\v{s} Pavunc, Andreja and Bani\'{c}, Martina and \v{C}uljak, Nina and Kazazi\'{c}, Sa\v{s}a and Kazazi\'{c}, Snje\v{z}ana and \v{S}u\v{s}kovi\'{c}, Jagoda and Kos, Bla\v{z}enka}, year = {2022}, pages = {111-111}, keywords = {breast milk microbiota, Levilactobacillus brevis, S-proteins}, isbn = {978-953-7778-18-7}, title = {The Role of S-Layer Proteins in Probiotic Properties of Levilactobacillus brevis Strains Isolated from Human Milk Microbiota}, keyword = {breast milk microbiota, Levilactobacillus brevis, S-proteins}, publisher = {Recedo digital j.d.o.o.}, publisherplace = {Sveti Martin na Muri, Hrvatska} }
@article{article, author = {Butorac, Katarina and Novak, Jasna and Lebo\v{s} Pavunc, Andreja and Bani\'{c}, Martina and \v{C}uljak, Nina and Kazazi\'{c}, Sa\v{s}a and Kazazi\'{c}, Snje\v{z}ana and \v{S}u\v{s}kovi\'{c}, Jagoda and Kos, Bla\v{z}enka}, year = {2022}, pages = {111-111}, keywords = {breast milk microbiota, Levilactobacillus brevis, S-proteins}, isbn = {978-953-7778-18-7}, title = {The Role of S-Layer Proteins in Probiotic Properties of Levilactobacillus brevis Strains Isolated from Human Milk Microbiota}, keyword = {breast milk microbiota, Levilactobacillus brevis, S-proteins}, publisher = {Recedo digital j.d.o.o.}, publisherplace = {Sveti Martin na Muri, Hrvatska} }




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