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Validation of Seratec Hemdirect on animal blood

The validation study of the SERATEC HemDirect blood test has been performed and the test is validated for forensic use. Initial validation test animal blood but all animal blood samples used in this study were from mammalian species and animals from other systematic groups were not included. This study aimed to determine whether the SERATEC HemDirect immunochromatographic test is highly specific for forensic identification of human blood, and whether there are non-mammalian species whose blood would show a false positive result on this test. The preliminary study included 8 samples of venous blood, human and 7 different animal species. All animal blood samples were obtained from Zagreb Zoo during the regular examination of animals. We used the blood of 7 animals who are commonly involved in car accidents or illegal hunting. All blood samples were analyzed by the SERATEC HemDirect test. A dilution set (1/4, 1/8, 1/10, 1/16, 1/20, 1/24) was prepared from whole blood by adding double- distilled water. A 50 µL sample of whole blood and each dilution were placed on a white cotton cloth and left to dry at room temperature. After drying, 1/4 of the sample was cut out and extracted in HemDirect buffer for 30 minutes. After extraction, three drops of each sample were added to the test well and the results were read and recorded after 10 minutes. The SERATEC HemDirect test showed positive results in all analyzed human blood samples (whole blood and dilutions 1/4, 1/8, 1/10, 1/16, 1/20, 1/24). In the analyzed blood samples of all animal species, the test gave negative results except in one case where a diluted blood sample of Rook (Corvus frugilegus L.) showed a weak positive result. The one weak positive result is ground for extending the study to more species of birds and reptiles since those can be found as pets in homes and can be involved in forensic investigations.

HemDirect ; validation ; forensics ; blood test

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