Gangliosidome of a human hippocampus in temporal lobe epilepsy resolved by high-resolution tandem mass spectrometry (CROSBI ID 311250)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Ica, Raluca ; Mlinac Jerković, Kristina ; Ilić, Katarina ; Sajko, Tomislav ; Munteanu, Cristian V. A. ; Zamfir, Alina D. ; Kalanj Bognar, Svjetlana
engleski
Gangliosidome of a human hippocampus in temporal lobe epilepsy resolved by high-resolution tandem mass spectrometry
In this study, we developed a high-resolution tandem mass spectrometry (HR MS) approach to assess presumed changes in gangliosidome of a human hippocampus affected by temporal lobe epilepsy (TLE) in comparison with a normal hippocampus. Gangliosides, membrane glycolipids, are particularly diverse and abundant in the human brain, and participate in ion transport and modulation of neuronal excitability. Changes in structural ganglioside pattern potentially linked to TLE molecular pathogenesis have not been explored in detail. Aiming to characterize TLE- specific gangliosidome, we analyzed the native gangliosides purified from a human hippocampal tissue sample affected by TLE and a control hippocampus using HR MS. Marked differences of ganglioside expression were shown in TLE vs. control, particularly with respect to the sialylation degree of components, discovered as a characteristic feature of TLE. Another major finding is the occurrence of tetrasialofucogangliosides in TLE and species modified by either O-acetylation or CH3COO−. Structural analysis by higher-energy collisional dissociation (HCD) MS/MS gave rise to fragmentation patterns implying that the GQ1b (d18:1/18:0) isomer is specifically associated with TLE. Further investigation in a larger sample is needed in order to confirm the discovery of ganglioside structures specifically expressed in human TLE and to provide information on the probable role of gangliosides in the molecular events underlying seizures.
gangliosides ; mass spectrometry ; temporal epilepsy ; human hippocampus
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Podaci o izdanju
Povezanost rada
Temeljne medicinske znanosti