engleski

Detection and characterisation of extended- spectrum and plasmid-mediated AmpC β-lactamase produced by Escherichia coli isolates found at poultry farms in Bosnia and Herzegovina

Extended spectrum beta-lactamases (ESBLs) are enzymes capable of conferring bacterial resistance to penicillins, first, second, third and fourth generation cephalosporins and aztreonam. Recently, ESBL producing organisms were identified in food producing animals as well. The aim of the study was to analyze ESBLs obtained from chicken farm in Zenica, Bosnia and Herzegovina. Antibiotic susceptibility was determined by disk- diffusion and broth microdilution method. ESBLs were detected by double-disk synergy test and inhibitor based test with clavulanic acid. Conjugation experiments were set up to determine the transferability of cefotaxime resistance. PCR was applied to detect the genes encoding ESBLs, plasmid-mediated AmpC beta-lactamases and quinolone resistance determinants. The isolates were unformly resistant to amoxycillin, cefazoline, cefotaxime and cefriaxone and unformly susceptible to imipenem, meropenem, ertapenem, amikacin and piperacillin/tazobactam. Eighteen isolates transferred cefotaxime resistance to E. coli recipient. blaCTX-M genes belonging to group 1 were detected by PCR in 21 isolates (77%) with 5 isolates being positive for blaCTX-M-15. Fourteen isolates (52%) tested positive for blaTEM genes. The most frequent incompatibility group was IncFIB in whereas IncFIA and Inc HI1 were present in a few isolates. Two different STs were identified: ST117 and ST155. The emergence of ESBL producing E. coli in farm animals possess a public health threat as they can cause intestinal colonization with subsequent infections in humans. The study emphasis the need for parallel studies on antimicrobial resistance in human and veterinary medicine.

antimicrobial resistance CTX-M ; ESBL ; chicken ; public health ST117 ; ST155

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