engleski

Microbial community comparison of two lake systems in Croatia

Freshwaters are not only the most important drinking water resources, but also sensitive indicators of environmental change. Lake ecosystems have been exposed to strong environmental and anthropogenic influence, making them highly vulnerable. Understanding the spatial and temporal variability of the microbial community in lakes is necessary to determine diversity patterns and monitor the environment. In this study, two lake systems with different climate, trophic status and lake type were investigated – Plitvice Lakes and Baćina Lakes. Samples were collected from May to November 2021. Environmental parameters were measured in situ using a multiparameter sonde (EXO2, YSI, USA). For taxonomic determination of the microbial community, the 16S rRNA pair-end sequencing method was used. Statistical analysis was performed using R software (R Core Team, 2021). The most abundant microbial communities in both lakes were Actinobacteriota, Bacteroidota, Cyanobacteria, Proteobacteria and Verrucomicrobiota. We determined seasonal changes in microbial communities in all lakes, but also identified some site characteristic communities: Fibrobacterota for Plitvice Lakes and the marine type phyla Nitrospinota, Nitrospirota and SAR324 for Baćina Lakes.

freshwater ecosystems ; amplicon sequencing ; seasonality

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