Influence of consumption of eggs enriched with functional compounds on endothelial function in healthy young subjects (CROSBI ID 718737)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Šušnjara, Petar ; Mihaljević, Zrinka ; Stupin, Ana ; Kolobarić, Nikolina ; Drenjančević, Ines
engleski
Influence of consumption of eggs enriched with functional compounds on endothelial function in healthy young subjects
Functional food, in addition to the appropriate nutritional effects, also has a beneficial effect on one or more targeted functions in the body, it improves health and wellbeing or reduces the risk of disease. Recent research within the Scientific Center of Excellence for Personalized Health Care has shown that consumption of n-3 polyunsaturated fatty acids (n-3 PUFAs) - fortified eggs had a positive effect on microvascular reactivity, and the level of oxidative stress and has mild anti- inflammatory effects in healthy young subjects of both sexes. The next step in functional food research is to further examine eggs enriched with other nutrients. The aim of this study was to determine the effect of consumption of hen eggs enriched with four nutrients (n-3 PUFA, lutein, vitamin E and selenium) on microvascular reactivity in healthy young subjects and to assess potential role of metabolites of COX1 and COX2 in that response. This was a randomised, double- blind, placebo-controlled interventional study (part of ID: NCT04564690, the study was registered on the clinicaltrial.gov). Subjects were divided in Control group (n=14 ; consumed regular hen eggs (approximately 1.785 mg of vitamin E, 0.330 mg of lutein, 0.054 mg of selenium and 438 mg of n-3 PUFAs daily), and Nutri4 group (n=20) who consumed enriched eggs (approximately 3.29 mg of vitamin E, 1.85 mg of lutein, 0.06 mg of selenium and 1026 mg of n-3 PUFAs daily), 3 eggs a day for 3 weeks. Skin microvascular blood flow in response to post- occlusive reactive hyperemia (PORH) and iontophoresis of acetylcholine and sodium nitroprusside (endothelium-independent) was assessed by laser Doppler flowmetry before and after dietary protocols. In separate sets of experiments, participants took perorally a 100 mg of indomethacin (a non-selective cyclooxygenase inhibitor) before functional (LDF) measurements. Protein expression of enzymes important in microvascular reactivity (COX1, COX2, iNOS, eNOS and nNOS) was assessed from peripheral blood mononuclear cells (PBMC) by Western blot before and after respective dietary protocol. Consumption of functionally enriched eggs improved endothelium-dependent vasodilation which was eliminated by indomethacin. The observed changes may be attributed to altered production of vasoactive metabolites by COX enzyme to which increased expression.
microcirculation, endothelium, vasodilation, cyclooxygenases, postocclusive hyperemic response, functional food, hen eggs
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Podaci o prilogu
19-20.
2022.
objavljeno
Podaci o matičnoj publikaciji
1847-2370
Podaci o skupu
27. međunarodno savjetovanje KRMIVA = 27th international conference KRMIVA
predavanje
01.06.2022-03.06.2022
Opatija, Hrvatska