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Optimization and Biotechnological Potential of Microalgae and Probiotic Strains in a Co-Cultivating System

Microalgae and their potential for food supplementation are a growing trend in modern society. Whole microalgal biomass and their extracts are rich in nutrients which give various health benefits. To further improve their characteristics as food supplements a co-cultivation with probiotic strains emerged as a new innovative way to increase nutrititional value. In order to increase probiotic strain acitivites, algae are usually added in powder form to cultivating broth. In this study, we performed co-cultivation of probiotic bacteria and viable microalga Chlorella vulgaris. Our main goal was to investigate if this approach can benefit to both organisms. We focused on optimization of growth conditions, examing inoculation ratio, temperatures, culturing volumes and media in correlation with biomass accumulation. Determination of C. vulgaris biomass composition has been performed using Nile Red (NR) method with flourescence microscopy for lipid accumulation ; spectrophotometry for pigments and Lowry method for protein yield. Metabolite products synthesized and released in culture medium, as well as metabolites embedded in biomass composition, were analysed using quadrupole time-of-flight (q-TOF) mass spectrometry revealing production of new compounds. Results of these comprehensive analysis will be presented.

Chlorella vulgaris ; Lactobacillus plantarum ; co-culture ; Q-TOF ; metabolites

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