engleski

Heat killed paraprobiotic Lactiplantibacillus plantarum S1 reduces DNA damage in induced oxidative stress

Oxidative stress is characterized by the inability of the organism to detoxify reactive oxygen species (ROS) and can be defined as a condition in which the balance between prooxidants and antioxidants in the cell is disturbed, leading to DNA hydroxylation, protein denaturation, lipid peroxidation, and cell apoptosis. The antioxidant effects of probiotic cells and probioticenriched foods have been previously noticed, but the exact mechanisms of antioxidant action are not fully understood. Literature suggests that probiotic therapy improves host oxidative status, which may lead to prevention of some chronic diseases. The concept of paraprobiotics has been proposed to refer to the use of inactivated microbial cells or cell fractions that provide health benefits to the host. Hence, we aimed to exert H2O2-induced oxidative stress on isolated human lymphocytes and investigate the protective effect of heat killed probiotic bacteria Lactiplantibacillus plantarum S1 and extracted bacterial metabolites smaller than 2.000 Daltons on nuclear DNA of isolated lymphocytes, using the comet assay. Both, metabolites and heat killed cells significantly reduced the H2O2-induced DNA damage (from 7.59±1.51% in induced stress to 4.07±1.61% for metabolites and 3.86±1.58% for heat killed cells), displaying a protective role. In combined treatment of heat killed cells and metabolites, the DNA damage was significantly lower (2.62±1.02%) than oxidative stress sample and the levels of primary DNA strand breaks did not differ compared to control sample (1.33±0.14%). Probiotic metabolites and heat-killed cells could potentially be used in a modified model to reduce the adverse effects associated with oxidative stress in the lower parts of the gastrointestinal tract.

Oxidative stress ; Inflammation ; Paraprobiotic ; Lactiplantibacillus ; DNA damage

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