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Pregled bibliografske jedinice broj: 1196211

Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles


Ledinski, Maja; Marić, Ivan; Peharec Štefanić, Petra; Ladan, Iva; Caput Mihalić, Katarina; Jurkin, Tanja; Gotić, Marijan; Urlić, Inga
Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles // Polymers, 14 (2022), 9; 1751, 11 doi:10.3390/polym14091751 (međunarodna recenzija, članak, znanstveni)


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Naslov
Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles

Autori
Ledinski, Maja ; Marić, Ivan ; Peharec Štefanić, Petra ; Ladan, Iva ; Caput Mihalić, Katarina ; Jurkin, Tanja ; Gotić, Marijan ; Urlić, Inga

Izvornik
Polymers (2073-4360) 14 (2022), 9; 1751, 11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ascorbate ; ascorbyl palmitate ; drug delivery ; cellular uptake ; nanoparticles ; antitumor effect

Sažetak
Antitumor applications of ascorbic acid (AA) and its oxidized form dehydroascorbic acid (DHA) can be quite challenging due to their instability and sensitivity to degradation in aqueous media. To overcome this obstacle, we have synthesized solid lipid nanoparticles loaded with ascorbyl palmitate (SLN-AP) with variations in proportions of the polymer Pluronic F-68. SLNs were synthesized using the hot homogenization method, characterized by measuring the particle size, polydispersity, zeta potential and visualized by TEM. To investigate the cellular uptake of the SLN, we have incorporated coumarin-6 into the same SLN formulation and followed their successful uptake for 48 h. We have tested the cytotoxicity of the SLN formulations and free ascorbate forms, AA and DHA, on HEK 293 and U2OS cell lines by MTT assay. The SLN-AP in both formulations have a cytotoxic effect at lower concentrations when compared to ascorbate applied the form of AA or DHA. Better selectivity for targeting tumor cell line was observed with 3% Pluronic F-68. The antioxidative effect of the SLN-AP was observed as early as 1 h after the treatment with a small dose of ascorbate applied (5 µM). SLN-AP formulation with 3% Pluronic F-68 needs to be further optimized as an ascorbate carrier due to its intrinsic cytotoxicity.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Projekti:
HRZZ-IP-2018-01-7590 - Selektivno ciljanje matičnih stanica sarkoma askorbinskom kiselinom (ASTar) (Urlić, Inga, HRZZ - 2018-01) ( POIROT)

Ustanove:
Hrvatski zavod za javno zdravstvo,
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com doi.org fulir.irb.hr

Citiraj ovu publikaciju:

Ledinski, Maja; Marić, Ivan; Peharec Štefanić, Petra; Ladan, Iva; Caput Mihalić, Katarina; Jurkin, Tanja; Gotić, Marijan; Urlić, Inga
Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles // Polymers, 14 (2022), 9; 1751, 11 doi:10.3390/polym14091751 (međunarodna recenzija, članak, znanstveni)
Ledinski, M., Marić, I., Peharec Štefanić, P., Ladan, I., Caput Mihalić, K., Jurkin, T., Gotić, M. & Urlić, I. (2022) Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles. Polymers, 14 (9), 1751, 11 doi:10.3390/polym14091751.
@article{article, author = {Ledinski, Maja and Mari\'{c}, Ivan and Peharec \v{S}tefani\'{c}, Petra and Ladan, Iva and Caput Mihali\'{c}, Katarina and Jurkin, Tanja and Goti\'{c}, Marijan and Urli\'{c}, Inga}, year = {2022}, pages = {11}, DOI = {10.3390/polym14091751}, chapter = {1751}, keywords = {ascorbate, ascorbyl palmitate, drug delivery, cellular uptake, nanoparticles, antitumor effect}, journal = {Polymers}, doi = {10.3390/polym14091751}, volume = {14}, number = {9}, issn = {2073-4360}, title = {Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles}, keyword = {ascorbate, ascorbyl palmitate, drug delivery, cellular uptake, nanoparticles, antitumor effect}, chapternumber = {1751} }
@article{article, author = {Ledinski, Maja and Mari\'{c}, Ivan and Peharec \v{S}tefani\'{c}, Petra and Ladan, Iva and Caput Mihali\'{c}, Katarina and Jurkin, Tanja and Goti\'{c}, Marijan and Urli\'{c}, Inga}, year = {2022}, pages = {11}, DOI = {10.3390/polym14091751}, chapter = {1751}, keywords = {ascorbate, ascorbyl palmitate, drug delivery, cellular uptake, nanoparticles, antitumor effect}, journal = {Polymers}, doi = {10.3390/polym14091751}, volume = {14}, number = {9}, issn = {2073-4360}, title = {Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles}, keyword = {ascorbate, ascorbyl palmitate, drug delivery, cellular uptake, nanoparticles, antitumor effect}, chapternumber = {1751} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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