Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1195817

The effect of ganglioside composition on enzyme activity, protein expression, and submembrane localization of Na+/K+ -ATPase in mouse brain


Puljko, Borna; Stojanović, Mario; Ilić, Katarina; Maček Hrvat, Nikolina; Heffer, Marija; Mlinac Jerković, Kristina; Kalanj Bognar, Svjetlana
The effect of ganglioside composition on enzyme activity, protein expression, and submembrane localization of Na+/K+ -ATPase in mouse brain // 17th International Symposium on Cholinergic Mechanisms (ISCM2022) - Programme and Abstracts / Kovarik, Zrinka ; Primožič, Ines (ur.).
Zagreb: Institute for Medical Research and Occupational Health, 2022. str. 71-71 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1195817 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The effect of ganglioside composition on enzyme activity, protein expression, and submembrane localization of Na+/K+ -ATPase in mouse brain

Autori
Puljko, Borna ; Stojanović, Mario ; Ilić, Katarina ; Maček Hrvat, Nikolina ; Heffer, Marija ; Mlinac Jerković, Kristina ; Kalanj Bognar, Svjetlana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
17th International Symposium on Cholinergic Mechanisms (ISCM2022) - Programme and Abstracts / Kovarik, Zrinka ; Primožič, Ines - Zagreb: Institute for Medical Research and Occupational Health, 2022, 71-71

ISBN
978-953-96817-8-2

Skup
17th International Symposium on Cholinergic Mechanisms (ISCM2022)

Mjesto i datum
Mlini, Dubrovnik, Hrvatska, 08-12.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
glycosphingolipids ; sodium potassium pump ; sodium potassium ATPase

Sažetak
The Na+ /K+ -ATPase (NKA) is an enzyme that asymmetrically distributes Na+ and K+ ions across the plasma membrane to generate and maintain the membrane potential, also having a role in signaling processes. NKA dysfunction has been implicated in several neurodegenerative disorders. Its positioning as well as different functions of active and inactive pools depend on interactions with neighboring membrane lipids. Ganglioside enriched lipid rafts (LR) are housing active NKA pool, while the bulk membrane contains the inactive pool. Gangliosides are known to modulate the structure, function and localization of membrane proteins thus having an impact on ion homeostasis. The aim of this study was to investigate the effect of altered ganglioside composition on activity, protein expression and submembrane localization of NKA, using St8sia1 null mice with impaired synthesis of gangliosides. Adult wild type (WT) and null mice littermates were sacrificed, brains neuroanatomically dissected and cortical and cerebellar homogenates prepared. NKA activity was measured spectrophotometrically. Protein expression of NKA in homogenates was analyzed by Western blotting. LR and non-raft (nLR) fractions from cortices and cerebella were isolated by ultracentrifugation in discontinuous sucrose gradients, and submembrane localization of NKA analyzed by Western blotting. Data revealed statistically lower NKA activity in cortices of null mice compared to the WT mice, whilst there was no disparity in the cerebella. Total protein amount of NKA was statistically lower in null mice cortices compared to their WTs, whilst it was unvaried in the cerebella. Analysis of submembrane localization has shown higher amount of NKA to be positioned within LR of cortices than those derived from the cerebella. These results demonstrate that altered ganglioside composition may contribute to lower NKA expression influencing NKA activity and cellular ion homeostasis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
IP-2016-06-8636 - Molekularni biljezi vulnerabilnosti, adaptacije i plastičnosti neurona u akutnoj i kroničnoj ozljedi mozga (NeuroReact) (Kalanj Bognar, Svjetlana, HRZZ - 2016-06) ( POIROT)
EK-EFRR-KK.01.1.1.01.0007 - Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi (Kostović, Ivica, EK - KK.01.1.1.01) ( POIROT)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Medicinski fakultet, Zagreb


Citiraj ovu publikaciju:

Puljko, Borna; Stojanović, Mario; Ilić, Katarina; Maček Hrvat, Nikolina; Heffer, Marija; Mlinac Jerković, Kristina; Kalanj Bognar, Svjetlana
The effect of ganglioside composition on enzyme activity, protein expression, and submembrane localization of Na+/K+ -ATPase in mouse brain // 17th International Symposium on Cholinergic Mechanisms (ISCM2022) - Programme and Abstracts / Kovarik, Zrinka ; Primožič, Ines (ur.).
Zagreb: Institute for Medical Research and Occupational Health, 2022. str. 71-71 (poster, međunarodna recenzija, sažetak, znanstveni)
Puljko, B., Stojanović, M., Ilić, K., Maček Hrvat, N., Heffer, M., Mlinac Jerković, K. & Kalanj Bognar, S. (2022) The effect of ganglioside composition on enzyme activity, protein expression, and submembrane localization of Na+/K+ -ATPase in mouse brain. U: Kovarik, Z. & Primožič, I. (ur.)17th International Symposium on Cholinergic Mechanisms (ISCM2022) - Programme and Abstracts.
@article{article, author = {Puljko, Borna and Stojanovi\'{c}, Mario and Ili\'{c}, Katarina and Ma\v{c}ek Hrvat, Nikolina and Heffer, Marija and Mlinac Jerkovi\'{c}, Kristina and Kalanj Bognar, Svjetlana}, year = {2022}, pages = {71-71}, keywords = {glycosphingolipids, sodium potassium pump, sodium potassium ATPase}, isbn = {978-953-96817-8-2}, title = {The effect of ganglioside composition on enzyme activity, protein expression, and submembrane localization of Na+/K+ -ATPase in mouse brain}, keyword = {glycosphingolipids, sodium potassium pump, sodium potassium ATPase}, publisherplace = {Mlini, Dubrovnik, Hrvatska} }
@article{article, author = {Puljko, Borna and Stojanovi\'{c}, Mario and Ili\'{c}, Katarina and Ma\v{c}ek Hrvat, Nikolina and Heffer, Marija and Mlinac Jerkovi\'{c}, Kristina and Kalanj Bognar, Svjetlana}, year = {2022}, pages = {71-71}, keywords = {glycosphingolipids, sodium potassium pump, sodium potassium ATPase}, isbn = {978-953-96817-8-2}, title = {The effect of ganglioside composition on enzyme activity, protein expression, and submembrane localization of Na+/K+ -ATPase in mouse brain}, keyword = {glycosphingolipids, sodium potassium pump, sodium potassium ATPase}, publisherplace = {Mlini, Dubrovnik, Hrvatska} }




Contrast
Increase Font
Decrease Font
Dyslexic Font