Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 119533

In vitro Embryo Production from Problem Cows by Transvaginal Ovum Pick-up


Getz, Iva; Lojkić, Martina; Samardžija, Marko; Matković, Mario; Makek, Zdenko; Cergolj, Marijan; Karadjole, Tugomir; Bačić, Goran
In vitro Embryo Production from Problem Cows by Transvaginal Ovum Pick-up // Reproduction in domestic animals, 38 (2003), 4. (podatak o recenziji nije dostupan, kongresno priopcenje, znanstveni)


Naslov
In vitro Embryo Production from Problem Cows by Transvaginal Ovum Pick-up

Autori
Getz, Iva ; Lojkić, Martina ; Samardžija, Marko ; Matković, Mario ; Makek, Zdenko ; Cergolj, Marijan ; Karadjole, Tugomir ; Bačić, Goran

Izvornik
Reproduction in domestic animals (0936-6768) 38 (2003), 4;

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
embryo; cow; ovarian stimulation; ovum pick-up; in vitro culture; SOFaaBSA

Sažetak
Ultrasound transvaginal ovum pick-up was performed in 4 Simmental cows suffering from reproductive disorders: cystic ovaries (n=3) and postpartum anoestrus due to endometritis (n=1). Rectal and ultrasound examination were performed during 1 month. Cows were treated with GnRH and PGF2a analogue. After resumption of normal cycle they were synchronized with PGF2a.Transvaginal ultrasound-guided oocyte collection was performed on day 3-4 of the cycle, twice a week for 4 consecutive weeks (OPU-A). After a recovery period of 2 consecutive oestrous cycles cows were stimulated with pFSH. Oocyte retrieval was performed every two weeks: 3-4 OPU sessions per cow (OPU-B). Oocytes were in vitro matured, fertilized and cultured. In non stimulated cows (OPU-A) the recovery rate was 43.6%, 1.9 COCs/OPU. In stimulated cows (OPU-B) recovery rate was 62.7%, 5.9 COCs/OPU. In vitro culture results for OPU-A and OPU-B were 76, 3% and 89% cleavage rate on Day 2, 54, 2% and 41.1% Morula/Blastocysts on Day 7 and 22% and 21.9% Hatched Blastocysts on Day 9 of culture respectively. The average number of embryos per OPU session were 1.84 and 4.87 Morula/Blastocysts and 0.41 and 1.01 Hatched Blastocysts per OPU-A and OPU-B respectively. More embryos were obtained in the FSH stimulated cows. There is no significant difference between problem cows and healthy donors from our previous research which indicated that problem cows could successfully be used for embryo production.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
0053339

Ustanove
Veterinarski fakultet, Zagreb

Profili:

Avatar Url Zdenko Makek (autor)

Avatar Url Goran Bačić (autor)

Avatar Url Mario Matković (autor)

Avatar Url Marijan Cergolj (autor)

Citiraj ovu publikaciju

Getz, Iva; Lojkić, Martina; Samardžija, Marko; Matković, Mario; Makek, Zdenko; Cergolj, Marijan; Karadjole, Tugomir; Bačić, Goran
In vitro Embryo Production from Problem Cows by Transvaginal Ovum Pick-up // Reproduction in domestic animals, 38 (2003), 4. (podatak o recenziji nije dostupan, kongresno priopcenje, znanstveni)
Getz, I., Lojkić, M., Samardžija, M., Matković, M., Makek, Z., Cergolj, M., Karadjole, T. & Bačić, G. (2003) In vitro Embryo Production from Problem Cows by Transvaginal Ovum Pick-up. Reproduction in domestic animals, 38 (4).
@article{article, year = {2003}, pages = {350}, keywords = {embryo, cow, ovarian stimulation, ovum pick-up, in vitro culture, SOFaaBSA}, journal = {Reproduction in domestic animals}, volume = {38}, number = {4}, issn = {0936-6768}, title = {In vitro Embryo Production from Problem Cows by Transvaginal Ovum Pick-up}, keyword = {embryo, cow, ovarian stimulation, ovum pick-up, in vitro culture, SOFaaBSA} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE