hrvatski

Ispitivanje ekotoksičnih učinaka nitromonoaromatskih spojeva in vitro i in vivo biotestovima

Nitromonoaromatic compounds (NACs) are toxic compounds that are ubiquitous in the atmosphere and may have mutagenic properties. They are considered to be indicators of secondary organic aerosol formed from anthropogenic pollution and biomass burning [1, 2, 3]. NACs represent a significant fraction of the water-soluble organic matter of aerosol that absorbs solar radiation and affects the overall radiative balance in the atmosphere [4]. Since NACs can enter the aquatic environment through atmospheric deposition, the aim of this study was to examine their ecotoxic effects on aquatic organisms. Model NACs (4-nitrophenol (4NP), 4-nitrocatechol (4NC), methyl-nitrocatechols (MNCs), 4-nitroguaiacol (4NG) and 2, 4-dinitrophenol (2, 4DNP) were analyzed by in vitro and in vivo bioassays. Acute cytotoxicity of the compounds was determined by MTT assay using PLHC-1 fish cells exposed for 72 hours to tested compounds. Chronic toxicity was determined by the AlgaeTox assay with the unicellular green algae Scenedesmus subspicatus after the 96 h exposure to the tested NACs. The ability of the compounds to induce phase I of the cellular detoxification mechanism was determined by the EROD bioassay by measuring the activity of CYP1A1 enzymes in fish PLHC-1 cells, while interaction with phase 0 was determined by measuring the inhibition of the transport activity of the zebrafish (Danio rerio) organic anion-transporting polypeptide Oatp1d1 stably expressed in the HEK293 Flp-In cell line. The obtained results show that some of the tested NACs showed a significant cytotoxic effect and the ability to inhibit protein transporter activity. The results of the EROD bioassay showed that the model NACs are not typical inducers of CYP1A1 detoxification enzyme. This research was financed by the Croatian Science Foundation project BiREADI (IP-2018-01-3105).

nitromonoaromatski spojevi ; toksičnost ; in vitro i in vivo biotestovi

nije evidentirano

engleski

Exploring ecotoxicological effects of nitromonoaromatic compounds by in vitro and in vivo bioassays

Nitromonoaromatic compounds (NACs) are toxic compounds that are ubiquitous in the atmosphere and may have mutagenic properties. They are considered to be indicators of secondary organic aerosol formed from anthropogenic pollution and biomass burning [1, 2, 3]. NACs represent a significant fraction of the water-soluble organic matter of aerosol that absorbs solar radiation and affects the overall radiative balance in the atmosphere [4]. Since NACs can enter the aquatic environment through atmospheric deposition, the aim of this study was to examine their ecotoxic effects on aquatic organisms. Model NACs (4-nitrophenol (4NP), 4-nitrocatechol (4NC), methyl-nitrocatechols (MNCs), 4-nitroguaiacol (4NG) and 2, 4-dinitrophenol (2, 4DNP) were analyzed by in vitro and in vivo bioassays. Acute cytotoxicity of the compounds was determined by MTT assay using PLHC-1 fish cells exposed for 72 hours to tested compounds. Chronic toxicity was determined by the AlgaeTox assay with the unicellular green algae Scenedesmus subspicatus after the 96 h exposure to the tested NACs. The ability of the compounds to induce phase I of the cellular detoxification mechanism was determined by the EROD bioassay by measuring the activity of CYP1A1 enzymes in fish PLHC-1 cells, while interaction with phase 0 was determined by measuring the inhibition of the transport activity of the zebrafish (Danio rerio) organic anion-transporting polypeptide Oatp1d1 stably expressed in the HEK293 Flp-In cell line. The obtained results show that some of the tested NACs showed a significant cytotoxic effect and the ability to inhibit protein transporter activity. The results of the EROD bioassay showed that the model NACs are not typical inducers of CYP1A1 detoxification enzyme. This research was financed by the Croatian Science Foundation project BiREADI (IP-2018-01-3105).

nitromonoaromatic compounds ; toxicity ; in vitro and in vivo bioassays

nije evidentirano