engleski

Bioaccessibility of the Fe, Mg and Zn from wheat grass in the human body: implementation of Caco-2 cellular model for intestinal absorption

Background: Wheatgrass is health supplement rich with vitamins, antioxidants, minerals, and proteins. Permeability data obtained by Caco-2 intestinal in vitro model correlate with human in vivo absorption mimicking transport through enterocyte membrane. The aim of this study is to evaluate bioaccessibility of minerals (Fe, Mg, Zn) indispensable for cells activity. Methods: The strain of wheat grass (Divana, Croatia) was evaluated in the form of fresh juice, digested juice, and powder before and after biofortification. Samples were collected every 15 minutes. Concentration in time dependant manner were estimated by AAS. Caco-2 model was confirmed by measurement of TEER value. Results: Results of absorption for analysed elements indicate different rate of absorption. After 45 minutes’ portion of absorbed elements are as follows: Fe (86.7 – 97.97 %), Mg (68.8 – 83.8 %) and Zn (65.7 – 81.53 %). The highest rate of absorption for Fe and Zn is observed after 15 minutes while absorption of magnesium is reached after 30 minutes. Conclusion: Results indicate passive model of transport for analysed minerals with the highs rate of absorption in the first 30 minutes indicating medium speed of absorption. Iron is better absorbed from biofortificated sample while magnesium and zinc are more effective absorbed from native sample.

humans ; Triticum ; biofortification ; minerals ; absorption ; Caco-2 cells

nije evidentirano