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Pregled bibliografske jedinice broj: 1194645

Selective interaction of microcystin congeners with zebrafish (Danio rerio) Oatp1d1 transporter


Marić, Petra; Ahel, Marijan; Maraković, Nikola; Lončar, Jovica; Mihaljević, Ivan; Smital, Tvrtko
Selective interaction of microcystin congeners with zebrafish (Danio rerio) Oatp1d1 transporter // Chemosphere, 283 (2021), 131155, 12 doi:10.1016/j.chemosphere.2021.131155 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1194645 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Selective interaction of microcystin congeners with zebrafish (Danio rerio) Oatp1d1 transporter

Autori
Marić, Petra ; Ahel, Marijan ; Maraković, Nikola ; Lončar, Jovica ; Mihaljević, Ivan ; Smital, Tvrtko

Izvornik
Chemosphere (0045-6535) 283 (2021); 131155, 12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Microcystins ; Zebrafish Oatp1d1 ; Kinetics determinations ; Substrates vs. inhibitors ; Chemicaly analytical determinations ; Molecular docking

Sažetak
Microcystins (MCs) are the most studied cyanotoxins. The uptake of MCs in cells and tissues of mammals and fish species is mostly mediated by organic anion-transporting polypeptides (OATPs in humans and rodents ; Oatps in other species), and the Oatp1d1 appears to be a major transporter for MCs in fish. In this study, six MC congeners of varying physicochemical properties (MC-LR, -RR, -YR, -LW, -LF, -LA) were tested by measuring their effect on the uptake of model Oatp1d1 fluorescent substrate Lucifer yellow (LY) in HEK293T cells transiently or stably overexpressing zebrafish Oatp1d1. MC-LW and -LF showed the strongest interaction resulting in an almost complete inhibition of LY transport with IC50 values of 0.21 and 0.26 μM, while congeners - LR, -YR and -LA showed lower inhibitory effects. To discern between Oatp1d1 substrates and inhibitors, results were complemented by Michaelis–Menten kinetics and chemical analytical determinations of MCs uptake, along with molecular docking studies performed using the developed zebrafish Oatp1d1 homology model. Our study showed that Oatp1d1-mediated transport of MCs could be largely dependent on their basic physicochemical properties, with log POW being the most obvious determinant. Finally, apart from determination of the chemical composition of cynobacterial blooms, a reliable risk assessment should take into account the interaction of identified MC congeners with Oatp1d1 as their primary transporter, and herewith we demonstrated that such a comprehensive approach could be based on the use of highly specific in vitro models, accompanied by chemical assessment and in silico molecular docking studies.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2019-04-1147 - Razumijevanje (eko)toksikološke uloge odabranih SLC i MATE transportnih proteina zebrice (Danio rerio) korištenjem metoda funkcionalne genomike (DANIOTRANS) (Smital, Tvrtko, HRZZ - 2019-04) ( POIROT)
EK-KF-KK.01.1.1.01.0003 - STIM-REI (STIM-REI) (Bonačić-Koutecky, Vlasta; Smital, Tvrtko; Orlić, Sandi, EK - KK.01.1.1.01) ( POIROT)
IP-2013-11-4307 - Dizajn, sinteza i evaluacija novih protuotrova kod trovanja živčanim bojnim otrovima i pesticidima (CHOLINESTERASE) (Kovarik, Zrinka, HRZZ - 2013-11) ( POIROT)
IP-2018-01-7683 - Analiza interakcija butirilkolinesteraze s novim inhibitorima i reaktivatorima (AnalyseBChE) (Kovarik, Zrinka, HRZZ - 2018-01) ( POIROT)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com doi.org

Citiraj ovu publikaciju:

Marić, Petra; Ahel, Marijan; Maraković, Nikola; Lončar, Jovica; Mihaljević, Ivan; Smital, Tvrtko
Selective interaction of microcystin congeners with zebrafish (Danio rerio) Oatp1d1 transporter // Chemosphere, 283 (2021), 131155, 12 doi:10.1016/j.chemosphere.2021.131155 (međunarodna recenzija, članak, znanstveni)
Marić, P., Ahel, M., Maraković, N., Lončar, J., Mihaljević, I. & Smital, T. (2021) Selective interaction of microcystin congeners with zebrafish (Danio rerio) Oatp1d1 transporter. Chemosphere, 283, 131155, 12 doi:10.1016/j.chemosphere.2021.131155.
@article{article, author = {Mari\'{c}, Petra and Ahel, Marijan and Marakovi\'{c}, Nikola and Lon\v{c}ar, Jovica and Mihaljevi\'{c}, Ivan and Smital, Tvrtko}, year = {2021}, pages = {12}, DOI = {10.1016/j.chemosphere.2021.131155}, chapter = {131155}, keywords = {Microcystins, Zebrafish Oatp1d1, Kinetics determinations, Substrates vs. inhibitors, Chemicaly analytical determinations, Molecular docking}, journal = {Chemosphere}, doi = {10.1016/j.chemosphere.2021.131155}, volume = {283}, issn = {0045-6535}, title = {Selective interaction of microcystin congeners with zebrafish (Danio rerio) Oatp1d1 transporter}, keyword = {Microcystins, Zebrafish Oatp1d1, Kinetics determinations, Substrates vs. inhibitors, Chemicaly analytical determinations, Molecular docking}, chapternumber = {131155} }
@article{article, author = {Mari\'{c}, Petra and Ahel, Marijan and Marakovi\'{c}, Nikola and Lon\v{c}ar, Jovica and Mihaljevi\'{c}, Ivan and Smital, Tvrtko}, year = {2021}, pages = {12}, DOI = {10.1016/j.chemosphere.2021.131155}, chapter = {131155}, keywords = {Microcystins, Zebrafish Oatp1d1, Kinetics determinations, Substrates vs. inhibitors, Chemicaly analytical determinations, Molecular docking}, journal = {Chemosphere}, doi = {10.1016/j.chemosphere.2021.131155}, volume = {283}, issn = {0045-6535}, title = {Selective interaction of microcystin congeners with zebrafish (Danio rerio) Oatp1d1 transporter}, keyword = {Microcystins, Zebrafish Oatp1d1, Kinetics determinations, Substrates vs. inhibitors, Chemicaly analytical determinations, Molecular docking}, chapternumber = {131155} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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